LOCUS       sequence-101103-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knock-in donor vector to insert Exon 44 in front of Exon 45 of human
            DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette 
            flanked by loxP sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Li HL, Fujimoto N, Sasakawa N, Shirai S, Ohkame T, Sakuma T, Tanaka 
            M, Amano N, Watanabe A, Sakurai H, Yamamoto T, Yamanaka S, Hotta A
  TITLE     Precise Correction of the Dystrophin Gene in Duchenne Muscular 
            Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and 
            CRISPR-Cas9.
  JOURNAL   Stem Cell Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 
            10.1016/j.stemcr.2014.10.013.
  PUBMED    25434822
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 
            10.1016/j.stemcr.2014.10.013."
COMMENT     Sequence Label: pENTR-DMD-Donor
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt
       61 cttataatgc caactttgta caagaaagct gggtctagat atctcgagtg cggccgcttg
      121 ctattgtgtc aaggagtagg agaatacaaa aatattagag aatatgaatc acatctatta
      181 ggagagtttt ctacatacgc acattattct gtcagtgaca taaggatttg agtcattcag
      241 atttaaatac ggtaggtacc tcaagttctc agatattatt tcattttcta aggttcgtat
      301 ttagttaata tgttatttta atggccttac aaattctaga ttatcttttt taaaaagtta
      361 aatagaacgt aattgccatt tttatttaat ggtaaaaagc atttttgttt ttgtgtgtac
      421 ttggttgtaa tattctcctt ttcaattgag ctatttttct gatactttac tcttaaaatt
      481 tcattcagga aaaaagtaaa caatatttaa gcttgacaat cataaaaatg ctctggtgac
      541 tatagattat tttaaaattt attactgtag cttagggata tcttgatggg atgctcctga
      601 aagcaattaa ttctcagttt tttgtggctt ctaatgcaaa atacattgac gcagacagaa
      661 tttgaaatga attttcttct aatatagcaa ttaattttat ttaaatatct ctagagtttt
      721 tttttaatac tgtgactaac ctatgtt
//
