LOCUS       sequence-101118-        1118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains two independent transcription units--a CMV 
            promoter/enhancer upstream of a MCS and a HSV thymidine kinase 
            promoter/enhancer upstream of the neomycin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Baker SJ, Markowitz S, Fearon ER, Willson JK, Vogelstein B
  TITLE     Suppression of human colorectal carcinoma cell growth by wild-type 
            p53.
  JOURNAL   Science. 1990 Aug 24. 249(4971):912-5.
  PUBMED    2144057
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1990 Aug 24. 249(4971):912-5."
COMMENT     Sequence Label: pCMV-Neo-Bam
FEATURES             Location/Qualifiers
     source          1..1118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          128..700
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(856..875)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    921..976
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(975..994)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cggtcgaccg atcctgagaa
      121 cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat
      181 gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta
      241 tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt
      301 gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt
      361 gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc
      421 actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac
      481 aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg
      541 aaatattctt attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg
      601 gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc
      661 ctctgctaac catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt
      721 tgttgtgctg tctcatcatt ttggcaaaga attcctcgac ggatccgtcg aggaattcac
      781 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
      841 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
      901 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
      961 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     1021 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     1081 ttggctataa agaggtcatc agtatatgaa acagcccc
//
