LOCUS       sequence-101119-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains two independent transcription units--a CMV 
            promoter/enhancer upstream of a MCS and a HSV thymidine kinase 
            promoter/enhancer upstream of the neomycin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Baker SJ, Markowitz S, Fearon ER, Willson JK, Vogelstein B
  TITLE     Suppression of human colorectal carcinoma cell growth by wild-type 
            p53.
  JOURNAL   Science. 1990 Aug 24. 249(4971):912-5.
  PUBMED    2144057
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1990 Aug 24. 249(4971):912-5."
COMMENT     Sequence Label: pCMV-Neo-Bam
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    133..154
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(169..174,175..192,193..199)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    207..223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     212..234
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     231..247
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     231..247
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     587..606
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    605..660
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     706..725
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
       61 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
      121 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
      181 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
      241 ctatgacatg attacgaatt gcggccgatc cgtcgaggga tctccataag agaagaggga
      301 cagctatgac tgggagtagt caggagagga ggaaaaatct ggctagtaaa acatgtaagg
      361 aaaattttag ggatgttaaa gaaaaaaata acacaaaaca aaatataaaa aaaatctaac
      421 ctcaagtcaa ggcttttcta tggaataagg aatggacagc agggggctgt ttcatatact
      481 gatgacctct ttatagccaa cctttgttca tggcagccag catatgggca tatgttgcca
      541 aactctaaac caaatactca ttctgatgtt ttaaatgatt tgccctccca tatgtccttc
      601 cgagtgagag acacaaaaaa ttccaacaca ctattgcaat gaaaataaat ttcctttatt
      661 agccagaagt cagatgctca aggggcttca tgatgtcccc ataatttttg gcagagggaa
      721 aaagatctca gtggtatttg tgagccaggg cattggccac accagccacc accttctgat
      781 aggcagcctg cacctgagga gtgaattcct cgacggatcc gtcgaggaat tctttgccaa
      841 aatgatgaga cagcacaaca accagcacgt tgcccaggag ctgtaggaaa gagaagaagg
      901 catgaacatg gttagcagag gggcccggtt tggactcaga gtattttatc ctcatctcaa
      961 acagtgtata tcattgtaac cataaagaga aaggcaggat gatgaccagg gtgtagttgt
     1021 ttctaccaat aa
//
