LOCUS       sequence-101125-       15963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP in neurons of transgenic zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15963)
  AUTHORS   Bai Q, Garver JA, Hukriede NA, Burton EA
  TITLE     Generation of a transgenic zebrafish model of Tauopathy using a 
            novel promoter element derived from the zebrafish eno2 gene.
  JOURNAL   Nucleic Acids Res. 2007;35(19):6501-16. Epub 2007 Sep 25.
  PUBMED    17897967
REFERENCE   2  (bases 1 to 15963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2007"; volume: "35(19)"; pages: "6501-16. Epub 
            2007 Sep 25"
COMMENT     Sequence Label: pBS-Isce1-eno2:egfp
FEATURES             Location/Qualifiers
     source          1..15963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(42..63)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(303..322)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     650..671
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    839..920
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(876..895)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     930..949
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(1095..1113)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1135..1151)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1135..1151)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1148..1170)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1159..1175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1183..1189,1190..1207,1208..1213))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1228..1249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1366..1383)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1537..2125)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1617..1636)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2296..3087,3088..3156))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2919..2938
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3157..3261)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3287..3742)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3424..3445)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3636..3655
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3869..3891
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3883..3900
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3884..3900
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3922..3941
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3922..3940
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(15960..15962,15963,1..2,3..716)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(15960..15962,15963,1..2,3..713)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
       61 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      121 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      181 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
      241 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      301 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      361 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
      421 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
      481 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
      541 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      601 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      661 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg
      721 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
      781 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
      841 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
      901 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatcaaa
      961 cacggccatt acggccaaac tcgctcgccc gggccgaacc agtggctgca gggccgcctc
     1021 ggccaaaggc ttaattaaag atctttacta gttctagagc ggccgccacc gcggtggagc
     1081 tccagctttt gttcccttta gtgagggtta attaccctgt tatccctaat catggtcata
     1141 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     1201 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     1261 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     1321 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     1381 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1441 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1501 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1561 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1621 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1681 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1741 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1801 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1861 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1921 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     1981 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2041 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2101 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2161 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2221 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2281 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2341 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2401 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2461 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2521 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2581 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2641 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2701 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2761 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2821 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2881 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2941 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3001 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3061 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3121 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3181 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3241 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
     3301 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     3361 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     3421 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     3481 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     3541 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     3601 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     3661 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     3721 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
     3781 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     3841 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     3901 gaaattaccc tgttatccct ataatacgac tcactatagg gcgaattggg tacctgttag
     3961 taaaggcaga ttcataataa caaaaaagta atttctttaa aagttacaga aacatcttgt
     4021 tgtatatcat cattaataaa tgagccaata tgtttccaaa attgtttaaa atggtagaat
     4081 tacacatgtt ctatatacac tgtacaacat atatgtcaat taaccttttt ttatttgtct
     4141 tgtgtttaca ttcactaagt ttgccagttg tttactcctt tggcattgtg ttatgtgacc
     4201 tttatgttta tgttaaaaat ggaactctgc agctcaacaa agtaattttt attgccatta
     4261 caccaatatg ttttataaga agaataaagt acaggcaatt tatatgacca acccagaaaa
     4321 tagactatat tgtttctaac tatactaaca atattaacaa aaaactttaa acatctaata
     4381 ctgcgattga aaagcataaa tcaacagaaa acactcctta atcacaaacg acatttttac
     4441 agaacgttta catttcatgt ggaaaatttt gggggcactt tataattttg aaactaatga
     4501 ttacttttgt cgatcaaacg tgccattaaa gatatttata actaaacagt gaatttctaa
     4561 ttcgtataat tgttgttttc aagttcacat gaatacaaca gaaggtaatg tggacttgaa
     4621 tgaattacac taactggcca ctttattagg tacacacgtc caactgctct ttaacacaaa
     4681 tttctaatca gccaatcacg tggcagcaac tcaatgcaac catgttattt aagtaaaagg
     4741 tgattaaagt gactttgaac gtggcatgtt tgttggcgcc agacgggctg gtcggagtat
     4801 ttcagaaatt gctgatcagt aactcaaata accactcgtt acaaccgagg tatgcagaag
     4861 agcatctctg aaaacacaac acgtccaacc ttgaggcaga tgggctacag cagcagaaga
     4921 tcacaccggg tgccactcct gtcacacctg aggctacaag tgacgctcac caaaattgga
     4981 caataggaaa aacattgcct gctctgatga gtctccattt ttctgctgca acattcgtat
     5041 ggtagggtca gaatttggca tcaacaacat gaaagcatgg atccatcctg ccttgtatca
     5101 atgattcagg ctgaagatgg tggtgtaatg gtgtggggga tattttcttg tgacactttg
     5161 ggcccattag tatcaactga gcatcatgta aatgccacag cctacctgag tattgtcgct
     5221 gaccatgtcc atctctttaa gaccacagtg tacccatctc ctgatggcta cttccagcag
     5281 gatatcgtac catgtcataa agcgtgaaac atctcagact ggtttcttaa acatgacaat
     5341 gaggtcactg tactcaaatg gcctccacag tcaccaggcc tcaatccaat agagcacctt
     5401 tggaatgtgg tggaatggga gatttgcatc atggatgtgc atccaacaaa tctgcagcaa
     5461 ctgtgtgatg ccatcatgtc aatatgacca aaatctctga ggaatatttc cagttcaatc
     5521 catgccacga aggattaagg cagctctaaa ggcataagcg ggtcctaacc tggtactagt
     5581 aaggtgtacc taataaagtg gctagtgtgt gtaagttact caaaaatatt agttttaaaa
     5641 cataaaagca gtctgtattc aaaatagtta cctcagctct tcggttttac agtgtttact
     5701 gacagaatca agcagcttta catgagtgac aggggtgttt ctctgaactg cacactgctg
     5761 tgtttttttg aagtacgact gcaaaagaaa tgtgacagtc tgtgactcta cagtgacccg
     5821 tctgctaaag tgaatttgac acatgctgaa aaaaaagcca tgactagaac ggaccacaca
     5881 tttgtttatt cagctcggct ctgatgtcaa caatgaaata tctttccttt aagaaagcca
     5941 tttcaaaaga gacgtaaaga cattgttgct cttattcgtt ttgaatactg ttggcaggtt
     6001 atacactggc taagttaata taaaaatgtt agattgtaat tgtaatttat atttaataat
     6061 aataataata ataatacatt ttatttatag gcgcctttct agcatctcaa ggacaccgta
     6121 caatcaacaa gagcctaaaa catcaagaga aataataaaa tatacacaac aatagtgcag
     6181 ataaaatgaa gtattaaaag ccatcttaaa taagtgggtt ttgagttttg atttgaatag
     6241 tgtgagggag tcaatgtttc tgatggcagg tggtaatgag ttccaaagcc gcggagcaga
     6301 gcggctaaat gctctgttgc ccatagttga aagacgagaa gagggaacag acaagtggag
     6361 ggaggaagaa gatcgcagag tgcgagaagg aacagagatg tggatgaggg cagacaaata
     6421 tggaggggcg agattgcgga tggctttgaa tgttaacatc aagattttaa agtcaattcg
     6481 aaaatgaaca ggtcatttaa attggtatac gttacactgt aaaaaaaaaa aaagccacac
     6541 aattcaggtc atcacaacac aactcaatta gttaacaaat ttaagtggat tgaacataaa
     6601 acaaccccaa caaaaaaatc tcaagaatcg tgttgattca gctcttttaa aaaatagttt
     6661 gaacaagctg caaaaatctt ttattttgtg taaagcaatt actatcagaa ttacaaaaaa
     6721 tcattcgggc aataaattga ctcaggtttc tttccaatga aagatgtgta ctctcaataa
     6781 ctcctctcaa aacactcttt ctttcacttt tctttcacaa acaacggagc aggggagaaa
     6841 tagcgaatat atctaaacat atcaacacgt gtaaactgta cataaatttg tgcgttatta
     6901 ataaattata ttaaataaaa tttgagctct ttcgatatct ttagtttcaa gcttaaacat
     6961 gtcctagata aaacctactg atttgaaggg tagcgtataa agatttaaaa agacaataaa
     7021 ttatttagca ctttcatcct taaatagggt cttttaatac gtcgtcaaat gaattataga
     7081 attttttttt tggtgtttaa atgaatgtta cactgagtga cattttagag gcatcatggt
     7141 aaaattgcat gatagtcatt ttttttatta ctgattaaaa ataaaataca agtactatta
     7201 ataatataaa aaaactacta ataactaaaa aataatatat gaataaaatt aactgagcac
     7261 attcgaagga acaaaatatg ttttttaaat gataatattg atattattta aatattaata
     7321 ttgttttgat gcttaaaacc cattttcatg ggttttcatg ggtccgtttt actttatttt
     7381 aagcattttt aacaagaaca aacattggct catactttac acgaacaaaa gacaatataa
     7441 aagaaaacaa caaagaaaag tgacaaaaaa cacacacaca cacacacaca cacacacatc
     7501 atagtcacaa aacatgcaat taaaactaga gagggtgtat agtttaaacc tcagaggggt
     7561 cagtggattg ctcttgagat atagatttgt caaataaatc aagaaattgt cctcaaatag
     7621 ccaaaatttt tctcgtggag ctagtcctta aaattctgag tctctctatc tgaatgacag
     7681 acatcatatc agaaatccag ttctggaatg aggggggggg gggggggggg taacagtgat
     7741 ttccaattat taagcaataa tcttttaacc accaccaggc cttgcattaa atagtcttgc
     7801 actgtttgag ggaaaatcca aagatagcaa gtttggcatc tggcagaaaa agtgtactat
     7861 aagccttgga gtaccagtca aatatgttgg tccagaatct gctcagtagg ggacagagcc
     7921 aaaaggcatg agcaaccgtg tcctctgcta ttttgcatct atcgcacatt ggtgagacag
     7981 aaggaaagat tttatggagc ttggctttag tatagtgtac acgatgtatg accttgaatt
     8041 aaaccagttg gtgtctgctg ttgactgagc atgactgtat tgaagtcaaa caattgtccc
     8101 atgtgtctgc agagagttcg attcccaaat ttttttcccc aggcaacttt aagtgaagtt
     8161 tttaaactaa tttcgagagg agcaagtgat ataattgact gcggctggcc acctatctac
     8221 actcattagt taaccaatca gatctatcct aactcactat aagtagccta gctagattta
     8281 ctcccttatc ttcgttttcc gaagaaaccc cccatccacc ccctttctcc tccttttctc
     8341 ctttacaaaa aaaagggagc tcttgagaac cacctgatct cgtactcccc tcacttgttc
     8401 tatggacctg gcgggagccc tgggctcaac tatctccgag ctcagggttc tctcccggga
     8461 cagcatgcca aacctgctta cagttgtcaa gcaatatcta agtgtgaact cttgaaatct
     8521 catggaggat attgtgacac aactttcaaa taaacacaga aatttataaa taagatgtct
     8581 ggaatcgggg gggggggggg gggggggggt gggggggggg gtgttgtaaa gatatcgtaa
     8641 aacacgtgat tttttggaca gagttcgaaa ttgggaattt tggacttcac aaaatgcctt
     8701 ggtttacaat catttaatac catttatatt gtttataata tattaataat ataatagtaa
     8761 tatatcatta aacacgctta aaaaaacttc tgcaatttgt cttgaaggtt tttcattctt
     8821 ctcttctgaa ctcatcaacc aacaagataa cataagtgaa atcaagttac agctggttct
     8881 ttagatcatt ctcattttaa agatgtgaca ttattccaat agtatctgga tgcagccttt
     8941 atgatgcaag ctgagattgc atcactcagc gatgcaatgt caggcacaat gcactcaaat
     9001 ggaacatgtg acgtcactgt gatgggtggg gttcggtgag cccattaaaa agcattggat
     9061 gcagctcaga ctgcatctgc accaggtctg catccagacc catctctgtt attctgcagt
     9121 cttagttatc tatagtggtt agcaacagtt acttcacagc aagaagattg ctggttcaag
     9181 ccctggctgg gttatttacg tgtttctgtg tggagtttgc atgttctcct tgtgttggtg
     9241 tgggtttcct ccggtttcca tcacagcccg aagacatgcg ctattgggga attaaataaa
     9301 ctaaaatggc tgtagtgttt gtgtgtaaat gcgagagtgt ataggtgttt cccagaactg
     9361 gattgcagct ggaagggcat ccagtgcata aaacatatgc tggaatagtt ggcggttcat
     9421 tccgctgtag tgaccgctga taaacaaagg gactaagccg aagaaaaact attggatgaa
     9481 cgtgatacat gtaaaagagt gaagttggtg taattgtatc tggctaagat gtttgtagtc
     9541 tgttgtcctc ataaccacct tctgtatctc agtctgaacg tatgtagtgt caagatcagc
     9601 gtgacccaat tctcagaaaa aataatctta ttaaaataaa cagcgtaatc acagattatc
     9661 tttcgctcac ctttctgcat cagtctcgta aacacagtct aacgtttcgt attcacttta
     9721 acaatataca tatttccgca caaaatcagc aattcagcat ttttactttt caaatgcgta
     9781 catttcaaac atatattatt acagtttact tcttagtaga atatggtaga ttgaaaatcc
     9841 aatagtttaa ttaagtcaac aacaatgtgt tttgtctttt gtaatgatct tggtttgtgt
     9901 gtaaaaaaat gtaatttgaa tgtgactatc cacaaggaat ttacaacatt gtaaaaagaa
     9961 attgctacat ttattaaccg aaaaatattt atgaataatt ttccacaatg ttgacatcag
    10021 cagagaatga aatgctgttt tcgagacaca gccactagaa aatcaacgga tgtttaccaa
    10081 aacaaaaatg tcttatagtt tgaaaaaata aaaagcatat gaagtattaa gaaaagtgaa
    10141 cagtcaattt tgattttacc gtttttttaa acactcaagt aaaatgtttt tatttcaaat
    10201 aatttttata ataaaaacta aatatttata aagtttcaca aagtaattta ttcattcatt
    10261 ttccttcggc ttagtccctt tattaatccg gggtcgccac agcggaatga tccgccaacc
    10321 catctctggg aaacatcaac acacacacac acacacacac tacggacaat ttagcctcgg
    10381 ctcagttggc gtttctgtgt ggagtttgca tgttctccct gccttcgcgt gggtttcccc
    10441 ctggtgctcc ggtttccccc acagtccaaa gacatgcggt gcaggtgaat tgggtaggct
    10501 aaattgtccg tagagtatga gcgtgtgtgt gaatgtgtgt gtggatgttt cccagtgatg
    10561 ggttgcggct ggaagggcat ccgttgtgta aaaacttgct ggataagttg gcggttcatt
    10621 ccgctgtggc gaccccggat taataaaggg actaagccga caagaaaatg aatgaatgaa
    10681 tgaatgaata tctgattttg tcattcagac catttttcaa ccaaaccttt atttgtccca
    10741 caactactca tgtttaagaa acaatttgat attttagaaa tttatgacag catatagtga
    10801 cattatacag tatatagtta aagtttaaat tattagccct ttgtgaattt ttctatattt
    10861 ttgaaaatat ttttcacatt ttttttcaca gtatttccaa taatattttt ttttcttcag
    10921 gagaaagtct tatttgtttt atttcagcta gaataaaagc agtttatata taaagcattt
    10981 taaggtcaat tctatttatt ttggattgtc tacaggacaa actattgtta tacaatgact
    11041 tgccttatta atctaagtta ggccattaaa ttgcacttta agctgaatac tagtatcttg
    11101 aaaaatatct agtcaaatat tatgtcctgt catcatggca aaaataaaag aaataagtta
    11161 ttaaaaccaa cacaggctca ttctgaaaac atagccctac tgtatataca tttctggaga
    11221 ttgcgaatta tgtagccaga agtaagtatg gctatatttc gtctttaaaa tgaacactag
    11281 ggggcgatat gatgccgttc cttttctcgc ttaccagctg accacttacc tcagtataga
    11341 cggctttccc gctgttacca gtttgtccaa tagctcgcgc atacatcgca gtcttgaaac
    11401 acagagaagg gttgactgcg ccgacggggt tcaagtccag tgaagaacgc ttccagaaag
    11461 caggtaagac ataaacaaaa gccaaaaata aaataaacaa gtaaataaca ttttgataat
    11521 gtaagtaaaa gctgaaaaca ttgtaaaaat caggcgaggg cttttctttt tctggattgc
    11581 ttttcaaaac actgtcggtt gggcttagga aagtgggtcg gcaagtcaat ttgagatctc
    11641 aaaaggcgta cacagcggcc tctggtggat tcgcataaac aaaaaactgc aaaaaaacat
    11701 atctcctggg atgttttggt gctctccaga aatgtatata ggggtacgtt ttcagaatga
    11761 gcctgggttg attcaaacta taatgtttag aaatgtataa aaatgaaaat ttaactggag
    11821 ggctaataat tctgaactgt atgtatacat tttttttatt gcaaaaaaac atcctgtaaa
    11881 ataacacatt ttaaaaatac accaaaaaca caaagttctg caacctaaaa tcctcattcc
    11941 accaacaata tgtttcaaca ttttcagcat tcgccactaa tgcaggcaca ttcagcatct
    12001 tttaaaccca gttcctccat gcatctattt ttaccagccg tcatgcacag aggagcattg
    12061 cagcaccctg tgtttgttgt ctcccctcct cttcccgggt cccttgttcc cgcttattaa
    12121 tcagtcgcct tgacctactc atcctcctgt ttctctcccc tctaattctc agcatcttcc
    12181 tctgactcat cttttcatcc cctccccctt ctgtttctcc tctcatctcg ctctcattct
    12241 gctttcctcc caccctcgct ctgttttcct ctctcttccg cagtttcaca cttccaggct
    12301 tgcctctcgc aaggctacac gcactgacgg caggtaagaa gcctttttat ttgacatcta
    12361 tttaggcagc tcatttccat catctgccat ggggtcattg aggtcattat gctaattatt
    12421 ctgtatacgg acttgatttg tgaatgttcc agttagaaaa ggaagacatc gactcgtgcg
    12481 tgaggtgtca cttcttatgt tgggatgctg gatttgattt tgggtggcgt tttttttttt
    12541 tttttggtgt ccgctggtag gctaaggggt cgggcagatg gatatcgcac tatgtgtttg
    12601 caacactctt tctgtattgc ctctcagagt gtgtgtgtgt gtgtgtgttg tcagtgtgtg
    12661 tccaatctgt tctaattcgt gatgtaagag tgggtcaatt tacagaattg ctcatgatac
    12721 atacagtttt gactaattta accaaagttt aatcgaaata ttacattttt tacccctaaa
    12781 tgaacacaaa tatgattaaa aatgcaacta tttatacagg aaaagataag aaacaaggaa
    12841 aaaatcgcga gtcgtacgga gttatttgta tattagtcac gtgacgcgcg cgaaaaaaaa
    12901 cacagaaacg aatcgctcgt gaacaaccca ctacatacta ctcgcttcga tgctccattt
    12961 cagaaagctc ttcttttcgc caaaggcatg ttaaaatatt tatcttgttc ttgccccatc
    13021 acgccacttt ctgcgctata tgcaccccac ccgtccgccc cctcctcccc ttatatctgc
    13081 tgcagtacca cacacactct ctctcacaca cacacattgg caaattagcc tcatccggat
    13141 ttagatgctc gatcataacc tgagcacctg cgggcaaaca ttccactgtg tcatcccgaa
    13201 taagggccct cctcctcggc cgctttcggt gctggtggtc gcccgttgca ttaacaaggt
    13261 gacacgtgcg cgcgcccatg tcggttgtag catatttagg atgcatttcg catgttctgc
    13321 ttttccattg gctgccagct caggcatctc catgtcgatg cttgagcagt ttctcccact
    13381 gctctacaaa agacgggact gaatgttcca gaaaaacaca gccgttttaa aaaggcacca
    13441 tcagattgat tgacatctcc tgacgcgttt gcagagatgc tgaagtccaa aaaaagatgg
    13501 cggtcacttc gtgtatgttt gtggggttat tttgtagaat taaagtgcat attttaaata
    13561 ttatataata ttcacagtct agccattttc cccatactat ttttccagtc acacgcaatt
    13621 ctttataaac cgtttgctat gactttgcct caataaactg ctaatttgct gcttattaat
    13681 agctagtagt tggttttaag tactaggtag gattagggat atgtaaaaaa gatcaacaca
    13741 gaacagaata tgtgctttat aagtactaat aaacaattat taaaccacta atagtgagaa
    13801 ttggtgctta aactacttat tttttattga atagcaaatg gtaataataa ttcctgtgaa
    13861 ttgtctagtc aagctgtcaa aatcccgaag cctaagggtg tgttcacact gcaagtttgg
    13921 tttaattaaa gtttggtaca caccaaggtg atggtcacct gtagtcattg tcctaatgac
    13981 tacgttacat ggacatcagt cattgatagg gctgggtgat tttaaaacga aaatcttgat
    14041 taattgaacg tcttaaatcg attacaatta atgaacgatt attttattta tttcctaatt
    14101 gtattttttg tcttcatagt tttatatgcc atcttaaaga atctctggcg cagcttccaa
    14161 tcatcgtcag tgtagtgcaa agtcaagaat gagtccatcg tcctactggt ccagagatca
    14221 gatgtggctg caaagtggct gcagaaatca gccacagcct ttaacagagt ggggtcacgt
    14281 gacacattaa gttatattgt tatattgaaa agttatattg attataggtt ctgaatgttg
    14341 acttcgattt cttttcgatt aatcgcccag tgatcaaatg atttgcctta atcgaaaaaa
    14401 atataatgat gatgtttaca tgagttgctt tttgaatgtt ccttttatac atgttatata
    14461 tcattataca gcaattaatg tcattgcctc agcccgctat ccatgtttcc tctggagttt
    14521 catgtaattt cgggtgtttt gtttttaatt tgttgacttt aactgcactt tggcactttc
    14581 actttcatct ggaacatttc atgtatgtcc gccctgacaa acaagatatt agatgtgtgg
    14641 aactgctgga agagtgtagt tttttttttt tttttgaaac ctttattgta taataagaca
    14701 gtagagagta ttgacaggaa agcatgggga gcagagagag gggaaggccg tgaggcagga
    14761 ttcaaactca ggtcgccgtg agcaccggtg tgtcgaccca ctaactaacc actgcaccac
    14821 tggcgccgac gaaaagtgta gttttaaagg aatttgatac catatgccga atagggaaac
    14881 aacctctgca tttcatgatg tgtgtgtctg tgttccttaa ctgcctcggt aggtgcagag
    14941 aagtgtcaaa cagttgtctg tgtatagaca aaatgcggtg atagtttgat tgtgttgttt
    15001 acatgtttgt actgcacttc aataacgaca gcgagagcat ctaagttcac tatggcgcac
    15061 ctgacaacaa cgatctggcc ctgagagcac caaaatttgc gacattactc atatttaaag
    15121 gggccgttgc agcatatcag caaatcacca acattcccgc atacaaatgt gctttctcgt
    15181 gtgaaaatgt tgcgcgcctt ttgtcaaatt catttaatac agaagatcca gttgtttgca
    15241 tgtgctgtgg ctttgctctt atccaatatg ttaatatgtc tgaaatattc tgaagcagca
    15301 atattgtttt gtactctcat tagttagcat aagattcccg tttaaaaaca aacatatata
    15361 acatttaatg cacatcagaa actcgccagt aaattttttt tatttaagac cctgtcagaa
    15421 aacattgtga acaattaaaa atccggcaac agcaatatta aaaacccttg ggaacaacag
    15481 tggtcagaac caaagttcac agggctgtgt tcagtggact tctctcaagt ggtggacctt
    15541 tacaatctgc catactatgg ctacaacagg cacactgata ttacagacgt ttcatttttc
    15601 gtccatcaca atgtatcagc agaagagtca agcttcaaat aatacaaact ctttcgccat
    15661 ttttgagtga catgctaatg gtctaatcgg attcaatgat gtatgctaag ctaagctaaa
    15721 agtgctcccg ccaaacctgt taaatgtctg aatggattaa aaaattataa aactgaactg
    15781 tttaacttta ggggagctgt aaaggaactt attttttaaa aagtggagtg ttactttaaa
    15841 ataaatgcca taaacagtgt ctaaacaaca cactgacatt gagaaaagta gaggtttagc
    15901 ttatcgtttg tctgtttttg ctcttgtctt taaggatgtc tgttgtaagc atcattgcca
    15961 tgg
//
