LOCUS       sequence-101126-        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP in neurons of transgenic zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Bai Q, Garver JA, Hukriede NA, Burton EA
  TITLE     Generation of a transgenic zebrafish model of Tauopathy using a 
            novel promoter element derived from the zebrafish eno2 gene.
  JOURNAL   Nucleic Acids Res. 2007;35(19):6501-16. Epub 2007 Sep 25.
  PUBMED    17897967
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2007"; volume: "35(19)"; pages: "6501-16. Epub 
            2007 Sep 25"
COMMENT     Sequence Label: pBS-Isce1-eno2:egfp
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    4..20
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9..31
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     28..44
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     28..44
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        66..84
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(230..249)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    259..340
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     284..303
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(508..529)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     857..876
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tagggataac
       61 agggtaatta accctcacta aagggaacaa aagctggagc tccaccgcgg tggcggccgc
      121 tctagaacta gtaaagatct ttaattaagc ctttggccga ggcggccctg cagccactgg
      181 ttcggcccgg gcgagcgagt ttggccgtaa tggccgtgtt tgatacattg atgagtttgg
      241 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
      301 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
      361 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
      421 caaatgtggt atggctgatt atgatctaga gtcgcggccg ctttacttgt acagctcgtc
      481 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
      541 ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag
      601 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
      661 gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc
      721 catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc
      781 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
      841 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
      901 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
      961 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
     1021 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
     1081 gcc
//
