LOCUS       sequence-101136-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a nuclear-targeted actin filament reporter, consisting of 
            the peptide Lifeact, on a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Belin BJ, Cimini BA, Blackburn EH, Mullins RD
  TITLE     Visualization of actin filaments and monomers in somatic cell 
            nuclei.
  JOURNAL   Mol Biol Cell. 2013 Apr;24(7):982-94. doi: 10.1091/mbc.E12-09-0685. 
            Epub 2013 Feb 27.
  PUBMED    23447706
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E12-09-0685"; journalName: "Mol Biol Cell"; date: 
            "2013-04"; volume: "24"; issue: "7"; pages: "982-94"
COMMENT     Sequence Label: pEGFP-C1 Lifeact-EGFP-2XNLS
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(152..171)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     499..520
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             599..619
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             623..643
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
       61 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      121 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      181 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      241 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
      301 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
      361 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
      421 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
      481 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
      541 cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt ccatggatcc
      601 aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtagatccaa aaagaagaga
      661 aagtgataat agggtaccgc gggcccggga tccaccggat ctagataact gatcataatc
      721 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
      781 aacctgaaac ataaaatgaa tgc
//
