LOCUS       sequence-101139-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nuclear-targeted non-polymerizing R62D mutant of human 
            actin, with an mCherry expression reporter after a P2A protease 
            cleavage site, on a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Belin BJ, Lee T, Mullins RD
  TITLE     DNA damage induces nuclear actin filament assembly by formin-2 and 
            Spire-1/2 that promotes efficient DNA repair.
  JOURNAL   Elife. 2015 Aug 19;4. doi: 10.7554/eLife.07735.
  PUBMED    26287480
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-08-19"; volume: "4. doi"; pages: " 10.7554/eLife.07735"
COMMENT     Sequence Label: pmCherry-C1 R62D actin-3XNLS P2A mCherry
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgatggatg atgatatcgc cgcgctcgtc gtcgacaacg
       61 gctccggcat gtgcaaggcc ggcttcgcgg gcgacgatgc cccccgggcc gtcttcccct
      121 ccatcgtggg gcgccccagg caccagggcg tgatggtggg catgggtcag aaggattcct
      181 atgtgggcga cgaggcccag agcaaggacg gcatcctcac cctgaagtac cccatcgagc
      241 acggcatcgt caccaactgg gacgacatgg agaaaatctg gcaccacacc ttctacaatg
      301 agctgcgtgt ggctcccgag gagcaccccg tgctgctgac cgaggccccc ctgaacccca
      361 aggccaaccg cgagaagatg acccagatca tgtttgagac cttcaacacc ccagccatgt
      421 acgttgctat ccaggctgtg ctatccctgt acgcctctgg ccgtaccact ggcatcgtga
      481 tggactccgg tgacggggtc acccacactg tgcccatcta cgaggggtat gccctccccc
      541 atgccatcct gcgtctggac ctggctggcc gggacctgac tgactacctc atgaagatcc
      601 tcaccgagcg cggctacagc ttcaccacca cggccgagcg ggaaatcgtg cgtgacatta
      661 aggagaagct gtgctacgtc gccctggact tcgagcaaga gatggccacg gctgcttcca
      721 gctcctccct ggagaagagc tacgagctgc ctgacggcca ggtcatcacc attggcaatg
      781 agcggttccg ctgccctgag gcactcttcc agccttcctt cctgggcatg gagtcctgtg
      841 gcatccacga aactaccttc aactccatca tg
//
