LOCUS       sequence-101140-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nuclear-targeted non-polymerizing R62D mutant of human 
            actin, with an mCherry expression reporter after a P2A protease 
            cleavage site, on a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Belin BJ, Lee T, Mullins RD
  TITLE     DNA damage induces nuclear actin filament assembly by formin-2 and 
            Spire-1/2 that promotes efficient DNA repair.
  JOURNAL   Elife. 2015 Aug 19;4. doi: 10.7554/eLife.07735.
  PUBMED    26287480
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-08-19"; volume: "4. doi"; pages: " 10.7554/eLife.07735"
COMMENT     Sequence Label: pmCherry-C1 R62D actin-3XNLS P2A mCherry
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             551..571
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             575..595
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             626..682
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      692..701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctacagcttc accaccacgg ccgagcggga aatcgtgcgt gacattaagg agaagctgtg
       61 ctacgtcgcc ctggacttcg agcaagagat ggccacggct gcttccagct cctccctgga
      121 gaagagctac gagctgcctg acggccaggt catcaccatt ggcaatgagc ggttccgctg
      181 ccctgaggca ctcttccagc cttccttcct gggcatggag tcctgtggca tccacgaaac
      241 taccttcaac tccatcatga agtgtgacgt ggacatccgc aaagacctgt acgccaacac
      301 agtgctgtct ggcggcacca ccatgtaccc tggcattgcc gacaggatgc agaaggagat
      361 cactgccctg gcacccagca caatgaagat caagatcatt gctcctcctg agcgcaagta
      421 ctccgtgtgg atcggcggct ccatcctggc ctcgctgtcc accttccagc agatgtggat
      481 cagcaagcag gagtatgacg agtccggccc ctccatcgtc caccgcaaat gcttcctacc
      541 ggtaatggat ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtagatcc
      601 aaaaaagaag agaaaggcgg ccgcagccac gaacttctct ctgttaaagc aagcaggaga
      661 cgtggaagaa aaccccggtc ctaaaccggt cgccaccatg gtgagcaagg gcgaggagga
      721 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
      781 cggccacgag ttcgagatcg a
//
