LOCUS       sequence-101141-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a nuclear-targeted mCherry control construct, on a CMV 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Belin BJ, Lee T, Mullins RD
  TITLE     DNA damage induces nuclear actin filament assembly by formin-2 and 
            Spire-1/2 that promotes efficient DNA repair.
  JOURNAL   Elife. 2015 Aug 19;4. doi: 10.7554/eLife.07735.
  PUBMED    26287480
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-08-19"; volume: "4. doi"; pages: " 10.7554/eLife.07735"
COMMENT     Sequence Label: pmCherry-C1 mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..727
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDTTSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(165..183)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     433..452
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tccgctagcg ctaccggtta tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
       61 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
      121 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      181 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
      241 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
      301 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
      361 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
      421 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
      481 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
      541 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
      601 gcccggcgcc tacaacgtca acatcaagtt ggacaccacc tcccacaacg aggactacac
      661 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
      721 gtacaagaga tctcgagctc aagcttccat ggat
//
