LOCUS       sequence-101142-         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a nuclear-targeted mCherry control construct, on a CMV 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Belin BJ, Lee T, Mullins RD
  TITLE     DNA damage induces nuclear actin filament assembly by formin-2 and 
            Spire-1/2 that promotes efficient DNA repair.
  JOURNAL   Elife. 2015 Aug 19;4. doi: 10.7554/eLife.07735.
  PUBMED    26287480
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-08-19"; volume: "4. doi"; pages: " 10.7554/eLife.07735"
COMMENT     Sequence Label: pmCherry-C1 mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     239..258
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             561..581
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             585..605
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
       61 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
      121 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
      181 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
      241 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
      301 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
      361 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
      421 acgtcaacat caagttggac accacctccc acaacgagga ctacaccatc gtggaacagt
      481 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagagatctc
      541 gagctcaagc ttccatggat ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa
      601 aggtagatcc aaaaaagaag agaaagtgat aatagggtac cgcgggcccg ggatccaccg
      661 gatctagata actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa
      721 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgc
//
