LOCUS       sequence-101146-         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a nuclear-targeted monomeric actin reporter, consisting of
            the RPEL1 domain from MAL/MRTF, on a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Belin BJ, Cimini BA, Blackburn EH, Mullins RD
  TITLE     Visualization of actin filaments and monomers in somatic cell 
            nuclei.
  JOURNAL   Mol Biol Cell. 2013 Apr;24(7):982-94. doi: 10.1091/mbc.E12-09-0685. 
            Epub 2013 Feb 27.
  PUBMED    23447706
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E12-09-0685"; journalName: "Mol Biol Cell"; date: 
            "2013-04"; volume: "24"; issue: "7"; pages: "982-94"
COMMENT     Sequence Label: pEGFP-C1 RPEL1-EGFP-3XNLS
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(114..133)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             561..581
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             585..605
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
       61 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      121 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      181 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      241 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
      301 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      361 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      421 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctt aaggagttcg
      481 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc
      541 gagctcaagc ttccatggat ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa
      601 aggtagatcc aaaaaagaag agaaagtgat aatagggtac cgcgggcccg ggatccaccg
      661 gatctagata actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa
      721 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgc
//
