LOCUS       sequence-101147-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a cytoplasmic actin filament reporter, the peptide 
            Lifeact, on a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Belin BJ, Goins LM, Mullins RD
  TITLE     Comparative analysis of tools for live cell imaging of actin network
            architecture.
  JOURNAL   Bioarchitecture. 2015 Aug 28:1-14.
  PUBMED    26317264
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Bioarchitecture. 2015 Aug 28:1-14."
COMMENT     Sequence Label: pEGFP-C1 Lifeact-EGFP
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..73
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(95..97,98..100,101..811)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLK
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(140..161)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(401..420)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcatgggcgt ggccgacctg atcaagaagt tcgagagcat
       61 cagcaaggaa gagggcgacc caccggtcgc caccatggtg agcaagggcg aggagctgtt
      121 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      181 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      241 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      301 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      361 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      421 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      481 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      541 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
      601 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      661 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
      721 caaagacccc aacgagaagc gcgatcacat ggtccttaag gagttcgtga ccgccgccgg
      781 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc
      841 gaattctgca gtcgacggta
//
