LOCUS       sequence-101150-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a nuclear-targeted eGFP on a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Belin BJ, Cimini BA, Blackburn EH, Mullins RD
  TITLE     Visualization of actin filaments and monomers in somatic cell 
            nuclei.
  JOURNAL   Mol Biol Cell. 2013 Apr;24(7):982-94. doi: 10.1091/mbc.E12-09-0685. 
            Epub 2013 Feb 27.
  PUBMED    23447706
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E12-09-0685"; journalName: "Mol Biol Cell"; date: 
            "2013-04"; volume: "24"; issue: "7"; pages: "982-94"
COMMENT     Sequence Label: pEGFP-C1 EGFP-3XNLS
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(190..209)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     537..558
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             637..657
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             661..681
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
       61 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
      121 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
      181 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      241 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      301 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
      361 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
      421 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      481 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
      541 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
      601 tccggactca gatctcgagc tcaagcttcc atggatccaa aaaagaagag aaaggtagat
      661 ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa agtgataata gggtaccgcg
      721 ggcccgggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
      781 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
      841 gc
//
