LOCUS       sequence-101152-        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Probe for HSPB12 for in situ hybridization in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Marvin M, O'Rourke D, Kurihara T, Juliano CE, Harrison KL, Hutson LD
  TITLE     Developmental expression patterns of the zebrafish small heat shock 
            proteins.
  JOURNAL   Dev Dyn. 2008 Feb;237(2):454-63.
  PUBMED    18161059
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn."; 
            date: "2008-02"; volume: "237(2)"; pages: "454-63"
COMMENT     Sequence Label: pCRII HSPB12
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(678..696)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(679..696)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(714..730)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(714..730)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(727..749)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    738..754
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(762..768,769..786,787..792))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    807..828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(945..962)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aattgtaata cgactcacta tagggcgaat tgggccctct agatgcatgc tcgagcggcc
       61 gccagtgtga tggatatctg cagaattcgc ccttacaacc tggagacgga catcagcgcc
      121 atcagcagca gcggacagga ccagacctgg gcggactcat tcactgatta ccaagtttac
      181 cagggagatc gagagtcttt tggaggttat gatgaagacc agaagtacat ccccggaaga
      241 aaccaggtag actggagcct cggagaccac atgaagactg gctccgaagt gggcgtggtg
      301 agagcagtgg gggaaaacta cttcctgtcg gcagacgtca gcggattcga gcctcatgaa
      361 gtggtcgtgc tggcgtatat cagtgtgtgg ttattcacgc agagaagagc ggatcagatg
      421 ttggtgtttc cggcaggttt acccacaagt ctgtgcttcc agcagacatg gacccgctgt
      481 cggtgagcag ttcactgacc tctgaccaga cgctgatcgt cagtatcaga cgaatcccaa
      541 aaacatcgag ctttgacctc ctgtgaaaat atatgaacct gtgtgtgaac gacgtgtgaa
      601 gggcgaattc cagcacactg gcggccgtta ctagtggatc cgagctcggt accaagcttg
      661 atgcatagct tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag
      721 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      781 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      841 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      901 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      961 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1021 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
//
