LOCUS       sequence-101153-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Probe for HSPB12 for in situ hybridization in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Marvin M, O'Rourke D, Kurihara T, Juliano CE, Harrison KL, Hutson LD
  TITLE     Developmental expression patterns of the zebrafish small heat shock 
            proteins.
  JOURNAL   Dev Dyn. 2008 Feb;237(2):454-63.
  PUBMED    18161059
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn."; 
            date: "2008-02"; volume: "237(2)"; pages: "454-63"
COMMENT     Sequence Label: pCRII HSPB12
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        22..40
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     22..39
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(693..712)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(694..712)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(719..736)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(719..735)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(728..750)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(963..982)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgaccatgat tacgccaagc tatttaggtg acactataga atactcaagc tatgcatcaa
       61 gcttggtacc gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgcccttc
      121 acacgtcgtt cacacacagg ttcatatatt ttcacaggag gtcaaagctc gatgtttttg
      181 ggattcgtct gatactgacg atcagcgtct ggtcagaggt cagtgaactg ctcaccgaca
      241 gcgggtccat gtctgctgga agcacagact tgtgggtaaa cctgccggaa acaccaacat
      301 ctgatccgct cttctctgcg tgaataacca cacactgata tacgccagca cgaccacttc
      361 atgaggctcg aatccgctga cgtctgccga caggaagtag ttttccccca ctgctctcac
      421 cacgcccact tcggagccag tcttcatgtg gtctccgagg ctccagtcta cctggtttct
      481 tccggggatg tacttctggt cttcatcata acctccaaaa gactctcgat ctccctggta
      541 aacttggtaa tcagtgaatg agtccgccca ggtctggtcc tgtccgctgc tgctgatggc
      601 gctgatgtcc gtctccaggt tgtaagggcg aattctgcag atatccatca cactggcggc
      661 cgctcgagca tgcatctaga gggcccaatt cgccctatag tgagtcgtat tacaattcac
      721 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      781 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      841 cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag
      901 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
      961 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     1021 tctaaatcgg gggctccctt tagggttc
//
