LOCUS       sequence-101154-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Probe for HSPB51b/15 for in situ hybridization in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Marvin M, O'Rourke D, Kurihara T, Juliano CE, Harrison KL, Hutson LD
  TITLE     Developmental expression patterns of the zebrafish small heat shock 
            proteins.
  JOURNAL   Dev Dyn. 2008 Feb;237(2):454-63.
  PUBMED    18161059
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn."; 
            date: "2008-02"; volume: "237(2)"; pages: "454-63"
COMMENT     Sequence Label: pCRII HSPB15
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        22..40
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     22..39
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             653..664
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(726..745)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(727..745)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(752..769)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(752..768)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(761..783)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(996..1015)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgaccatgat tacgccaagc tatttaggtg acactataga atactcaagc tatgcatcaa
       61 gcttggtacc gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgcccttt
      121 tcagaacgca gacagaacct tgtaattcca aggtgacaat ttgttaaact gattatgttt
      181 acccagtaac tactgagagt ctgaatgtct ctggttactg gtgaggcata gttcatccgt
      241 ttttgcaggt aaccgtttgt ccatcatgtg aatagggatg acaatctctc ctgggaaact
      301 gatattagag cccgttaatg gagcttcaac tgagagaaca ccatctggag acagcatgga
      361 gctgatctgt tttaggtcta agtcagccgg aagcttgtat tttcttgcaa agcttcttga
      421 gatcagcctg tgattttcct gtctttcctc gtgattacct gttatttcca agtatccgtc
      481 cctggttttg acgcttatct cttctggaga aaaaggaccg acatctagac aaactttcca
      541 gtcttgctct cctatcattt gttcacacac agcatcagaa acgtgtcctt cagatccagt
      601 acatgattta gatggaatct ccttgcacaa tgagctgaag agaggaaaag gcatcgaagg
      661 gcgaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct agagggccca
      721 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
      781 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      841 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      901 gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      961 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     1021 tctcgccacg
//
