LOCUS       sequence-101155-        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Probe for HSPB51b/15 for in situ hybridization in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Marvin M, O'Rourke D, Kurihara T, Juliano CE, Harrison KL, Hutson LD
  TITLE     Developmental expression patterns of the zebrafish small heat shock 
            proteins.
  JOURNAL   Dev Dyn. 2008 Feb;237(2):454-63.
  PUBMED    18161059
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn."; 
            date: "2008-02"; volume: "237(2)"; pages: "454-63"
COMMENT     Sequence Label: pCRII HSPB15
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(88..99)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(712..730)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(713..730)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(748..764)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(748..764)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(761..783)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    772..788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(796..802,803..820,821..826))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    841..862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(979..996)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaattgtaat acgactcact atagggcgaa ttgggccctc tagatgcatg ctcgagcggc
       61 cgccagtgtg atggatatct gcagaattcg cccttcgatg ccttttcctc tcttcagctc
      121 attgtgcaag gagattccat ctaaatcatg tactggatct gaaggacacg tttctgatgc
      181 tgtgtgtgaa caaatgatag gagagcaaga ctggaaagtt tgtctagatg tcggtccttt
      241 ttctccagaa gagataagcg tcaaaaccag ggacggatac ttggaaataa caggtaatca
      301 cgaggaaaga caggaaaatc acaggctgat ctcaagaagc tttgcaagaa aatacaagct
      361 tccggctgac ttagacctaa aacagatcag ctccatgctg tctccagatg gtgttctctc
      421 agttgaagct ccattaacgg gctctaatat cagtttccca ggagagattg tcatccctat
      481 tcacatgatg gacaaacggt tacctgcaaa aacggatgaa ctatgcctca ccagtaacca
      541 gagacattca gactctcagt agttactggg taaacataat cagtttaaca aattgtcacc
      601 ttggaattac aaggttctgt ctgcgttctg aaaagggcga attccagcac actggcggcc
      661 gttactagtg gatccgagct cggtaccaag cttgatgcat agcttgagta ttctatagtg
      721 tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      781 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      841 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      901 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      961 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1021 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gg
//
