LOCUS       sequence-101156-        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Probe for HSPB9 for in situ hybridization in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Marvin M, O'Rourke D, Kurihara T, Juliano CE, Harrison KL, Hutson LD
  TITLE     Developmental expression patterns of the zebrafish small heat shock 
            proteins.
  JOURNAL   Dev Dyn. 2008 Feb;237(2):454-63.
  PUBMED    18161059
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn."; 
            date: "2008-02"; volume: "237(2)"; pages: "454-63"
COMMENT     Sequence Label: pCRII HSPB9
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        22..40
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     22..39
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(726..745)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(727..745)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(752..769)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(752..768)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(761..783)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(996..1015)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgaccatgat tacgccaagc tatttaggtg acactataga atactcaagc tatgcatcaa
       61 gcttggtacc gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccctta
      121 atcgagggat gtcctgaagc ggatgggaac gacgtgctcc tccgaggact cgtctttggt
      181 ttcttctggt gtctcgatat gcaggagtcc atcttcacca agtgagcagg tcaatgaagc
      241 tggatccagg tgatcaggaa gctgcacggc ctgaacaaac tcttgagcct gggactcagc
      301 agatgacgaa gaagcgtttt tctcagccct ctttccggcc atcacctcca gccttcgtcc
      361 agacaccgtg acactcacat cctctggaga aaagcctcgg gtgtccagag taagagacac
      421 gtcctgcttc ttcttctgct ctgggtcagt gctgaacagc ctggagaagg ttgaggaacc
      481 atctagagtt tggaagaaat cttcacaaag ttcattcagc aaactgtctc tgatctcact
      541 cctcaggttc tgcattattt gggctctacg gtggagaaaa tcctctctga agctggaaag
      601 aggcatgccg cgtctgggcc gcaagaagta ggagttctca aagaaagggt cgtccaaagg
      661 gcgaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct agagggccca
      721 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
      781 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      841 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      901 gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      961 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     1021 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
//
