LOCUS       sequence-101157-        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Probe for HSPB9 for in situ hybridization in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Marvin M, O'Rourke D, Kurihara T, Juliano CE, Harrison KL, Hutson LD
  TITLE     Developmental expression patterns of the zebrafish small heat shock 
            proteins.
  JOURNAL   Dev Dyn. 2008 Feb;237(2):454-63.
  PUBMED    18161059
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn."; 
            date: "2008-02"; volume: "237(2)"; pages: "454-63"
COMMENT     Sequence Label: pCRII HSPB9
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(712..730)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(713..730)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(748..764)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(748..764)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(761..783)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    772..788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(796..802,803..820,821..826))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    841..862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(979..996)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaattgtaat acgactcact atagggcgaa ttgggccctc tagatgcatg ctcgagcggc
       61 cgccagtgtg atggatatct gcagaattcg ccctttggac gaccctttct ttgagaactc
      121 ctacttcttg cggcccagac gcggcatgcc tctttccagc ttcagagagg attttctcca
      181 ccgtagagcc caaataatgc agaacctgag gagtgagatc agagacagtt tgctgaatga
      241 actttgtgaa gatttcttcc aaactctaga tggttcctca accttctcca ggctgttcag
      301 cactgaccca gagcagaaga agaagcagga cgtgtctctt actctggaca cccgaggctt
      361 ttctccagag gatgtgagtg tcacggtgtc tggacgaagg ctggaggtga tggccggaaa
      421 gagggctgag aaaaacgctt cttcgtcatc tgctgagtcc caggctcaag agtttgttca
      481 ggccgtgcag cttcctgatc acctggatcc agcttcattg acctgctcac ttggtgaaga
      541 tggactcctg catatcgaga caccagaaga aaccaaagac gagtcctcgg aggagcacgt
      601 cgttcccatc cgcttcagga catccctcga ttaagggcga attccagcac actggcggcc
      661 gttactagtg gatccgagct cggtaccaag cttgatgcat agcttgagta ttctatagtg
      721 tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      781 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      841 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      901 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      961 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1021 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gg
//
