LOCUS       sequence-101166-        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Probe for HSPB8 for in situ hybridization in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Marvin M, O'Rourke D, Kurihara T, Juliano CE, Harrison KL, Hutson LD
  TITLE     Developmental expression patterns of the zebrafish small heat shock 
            proteins.
  JOURNAL   Dev Dyn. 2008 Feb;237(2):454-63.
  PUBMED    18161059
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn."; 
            date: "2008-02"; volume: "237(2)"; pages: "454-63"
COMMENT     Sequence Label: pCRII HSPB8
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(937..955)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(938..955)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(973..989)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(973..989)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(986..1008)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    997..1013
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1021..1027,1028..1045,1046..1051))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gaattgtaat acgactcact atagggcgaa ttgggccctc tagatgcatg ctcgagcggc
       61 cgccagtgtg atggatatct gcagaattcg cccttttgca cacttccctt gtcacttgat
      121 tattagctct atcacattaa cttattttca ttgatgttgt atttgaccta accagataca
      181 gtagagtaac ttttgatgat ttatggacta tttgctttca gattaatgac tttttgattt
      241 aagtatatgt ttgaaactga taaacctaga ttgtagcgcg ggcttggaac aaataagagt
      301 tgcgcaatct cccgttcact ccctggcctc aaaggttgtg gttgaagtca tgctgggctc
      361 ctgaggtctg gcctcaggtt cttccattga ttctgctggc atttcattgc tgtagaggta
      421 gtacggaggg gtttgtcggg cttcaatgat gagaacccct tccggagata gagaggcaaa
      481 cacagtgaca gggtccacat ccaatgggat ttgaattttc tttgtgaagt ttttggtaac
      541 aattcctccc tcgtcttgct tttcctcatg tttccctgac acctctacaa agccgtcttt
      601 agttttgaca ttaagctctt cgggcttgaa gctgtgcacg tttacgcaca ctttccacgg
      661 ctcgtccgag tcggtcgggg gcgcgctggc tcgtcgtggg ctctcggtgt acacagagct
      721 gaagccctgc ggggagctca tactcgcccg cgggaagccc gagcgcaaag agcccgtcca
      781 cggggcgtcc aggcggtggc tcaggcgagg ccgagcccac ccgggccaat ccatggacaa
      841 ctcattggga aagggtgaag ggcgaattcc agcacactgg cggccgttac tagtggatcc
      901 gagctcggta ccaagcttga tgcatagctt gagtattcta tagtgtcacc taaatagctt
      961 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     1021 caacatacga gccggaagca taaagtgtaa agcctggg
//
