LOCUS       sequence-101168-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Probe for HSPB8 for in situ hybridization in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Marvin M, O'Rourke D, Kurihara T, Juliano CE, Harrison KL, Hutson LD
  TITLE     Developmental expression patterns of the zebrafish small heat shock 
            proteins.
  JOURNAL   Dev Dyn. 2008 Feb;237(2):454-63.
  PUBMED    18161059
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn."; 
            date: "2008-02"; volume: "237(2)"; pages: "454-63"
COMMENT     Sequence Label: pCRII HSPB8
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        22..40
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     22..39
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(951..970)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(952..970)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(977..994)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(977..993)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(986..1008)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgaccatgat tacgccaagc tatttaggtg acactataga atactcaagc tatgcatcaa
       61 gcttggtacc gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgcccttc
      121 accctttccc aatgagttgt ccatggattg gcccgggtgg gctcggcctc gcctgagcca
      181 ccgcctggac gccccgtgga cgggctcttt gcgctcgggc ttcccgcggg cgagtatgag
      241 ctccccgcag ggcttcagct ctgtgtacac cgagagccca cgacgagcca gcgcgccccc
      301 gaccgactcg gacgagccgt ggaaagtgtg cgtaaacgtg cacagcttca agcccgaaga
      361 gcttaatgtc aaaactaaag acggctttgt agaggtgtca gggaaacatg aggaaaagca
      421 agacgaggga ggaattgtta ccaaaaactt cacaaagaaa attcaaatcc cattggatgt
      481 ggaccctgtc actgtgtttg cctctctatc tccggaaggg gttctcatca ttgaagcccg
      541 acaaacccct ccgtactacc tctacagcaa tgaaatgcca gcagaatcaa tggaagaacc
      601 tgaggccaga cctcaggagc ccagcatgac ttcaaccaca acctttgagg ccagggagtg
      661 aacgggagat tgcgcaactc ttatttgttc caagcccgcg ctacaatcta ggtttatcag
      721 tttcaaacat atacttaaat caaaaagtca ttaatctgaa agcaaatagt ccataaatca
      781 tcaaaagtta ctctactgta tctggttagg tcaaatacaa catcaatgaa aataagttaa
      841 tgtgatagag ctaataatca agtgacaagg gaagtgtgca aaagggcgaa ttctgcagat
      901 atccatcaca ctggcggccg ctcgagcatg catctagagg gcccaattcg ccctatagtg
      961 agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctgg
//
