LOCUS       sequence-101172-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Ward JD
  TITLE     Rapid and Precise Engineering of the Caenorhabditis elegans Genome 
            with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair.
  JOURNAL   Genetics. 2014 Dec 9. pii: genetics.114.172361.
  PUBMED    25491644
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 Dec 9. pii: genetics.114.172361."
COMMENT     Sequence Label: pJW1219
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..605
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
ORIGIN
        1 agttggcacc tttggtcttt tattgtcaac ttccattggt tcttccattg tttctgttaa
       61 attaatgaat ttttcataaa ataaagacat tatacaatat aaaaatgaag aatttattga
      121 aaataaactg ccagagagaa aaagtatgca acactcccgc cgagagtgtt tgaaatggtg
      181 tacggtacat tttcgtgcta ggagttagat gtgcaggcag caacgagagg gggagagatt
      241 tttttgggcc ttgtgaaatt aacgtgagtt ttctggtcat ctgactaatc atgttggttt
      301 tttgttggtt tattttgttt ttatctttgt ttttatccag attaggaaat ttaaatttta
      361 tgaatttata atgaggtcaa acattcagtc ccagcgtttt tcctgttctc actgtttagt
      421 cgaattttta ttttaggctt tcaacaaatg ttctaactgt cttatttgtg acctcacttt
      481 ttatattttt ttaattttta aaaatattag aagtttctag gataattttt tcgactttta
      541 ttctctctac cgtccgcact cttcttactt ttaaattaaa ttgttttttt ttcagttggg
      601 aaacactttg ctctagaaaa aatggacaaa aaatacagca tcggccttga catcggaacg
      661 aactccgtcg gatgggccgt catcaccgac gagtacaagg tcccatccaa gaagttcaag
      721 gtcctcggaa acaccgaccg tcactccatc aagaagaacc tcatcggagc cctcctcttc
      781 gactccggag agaccgccga ggccacccgt ctcaagcgta ccgcccgtcg tcgttacacc
      841 cgtcgtaaga accgtatctg ctacctccaa gagatcttct c
//
