LOCUS       sequence-101173-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 plasmid with sgRNA(F+E) targeting pha-1; for 
            co-conversion in C. elegans .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Ward JD
  TITLE     Rapid and Precise Engineering of the Caenorhabditis elegans Genome 
            with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair.
  JOURNAL   Genetics. 2014 Dec 9. pii: genetics.114.172361.
  PUBMED    25491644
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 Dec 9. pii: genetics.114.172361."
COMMENT     Sequence Label: pJW1285
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..604
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
ORIGIN
        1 gttggcacct ttggtctttt attgtcaact tccattggtt cttccattgt ttctgttaaa
       61 ttaatgaatt tttcataaaa taaagacatt atacaatata aaaatgaaga atttattgaa
      121 aataaactgc cagagagaaa aagtatgcaa cactcccgcc gagagtgttt gaaatggtgt
      181 acggtacatt ttcgtgctag gagttagatg tgcaggcagc aacgagaggg ggagagattt
      241 ttttgggcct tgtgaaatta acgtgagttt tctggtcatc tgactaatca tgttggtttt
      301 ttgttggttt attttgtttt tatctttgtt tttatccaga ttaggaaatt taaattttat
      361 gaatttataa tgaggtcaaa cattcagtcc cagcgttttt cctgttctca ctgtttagtc
      421 gaatttttat tttaggcttt caacaaatgt tctaactgtc ttatttgtga cctcactttt
      481 tatatttttt taatttttaa aaatattaga agtttctagg ataatttttt cgacttttat
      541 tctctctacc gtccgcactc ttcttacttt taaattaaat tgtttttttt tcagttggga
      601 aacactttgc tctagaaaaa atggacaaaa aatacagcat cggccttgac atcggaacga
      661 actccgtcgg atgggccgtc atcaccgacg agtacaaggt cccatccaag aagttcaagg
      721 tcctcggaaa caccgaccgt cactccatca agaagaacct catcggagcc ctcctcttcg
      781 actccggaga gaccgccgag gccacccgtc tcaagcgtac cgcccgtcgt cgttacaccc
      841 gtcgtaagaa ccgtatctgc tacctccaag agatcttctc caacga
//
