LOCUS       sequence-101177-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by 
            PCR-fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Ward JD
  TITLE     Rapid and Precise Engineering of the Caenorhabditis elegans Genome 
            with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair.
  JOURNAL   Genetics. 2014 Dec 9. pii: genetics.114.172361.
  PUBMED    25491644
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 Dec 9. pii: genetics.114.172361."
COMMENT     Sequence Label: pJW1311
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        28..46
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     28..45
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     129..147
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(185..207)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(678..697)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(679..697)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac tcaagctatg
       61 catcaagctt ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattcg
      121 cccttaaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
      181 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
      241 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
      301 actatgcggc atcagagcag attgcactga gagtgcacca tatgcggtgt gaaataccgc
      361 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
      421 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagaaattt cacaaaaaaa
      481 agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt taaacttgct
      541 atgctgtttc cagcatagct cttaaactca gggtttcagc ctcgttcaag acatctcgca
      601 ataggaggaa gggcgaattc tgcagatatc catcacactg gcggccgctc gagcatgcat
      661 ctagagggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc g
//
