LOCUS       sequence-101181-         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Ward JD
  TITLE     Rapid and Precise Engineering of the Caenorhabditis elegans Genome 
            with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair.
  JOURNAL   Genetics. 2014 Dec 9. pii: genetics.114.172361.
  PUBMED    25491644
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 Dec 9. pii: genetics.114.172361."
COMMENT     Sequence Label: pJW1219
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..19
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(57..79)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        complement(460..807)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
ORIGIN
        1 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
       61 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
      121 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
      181 gcatcagagc agattgcact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
      241 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
      301 gggcgatcgg tgcgggcctc ttcgctatta cgccagaaat ttcacaaaaa aaagcaccga
      361 ctcggtgcca ctttttcaag ttgataacgg actagcctta tttaaacttg ctatgctgtt
      421 tccagcatag ctcttaaaca attgactaca catccatcca agacatctcg caataggagg
      481 tgtttatata ggggcaacac aagaggcggc agacatctcg tgagtttgac agagtagtat
      541 aaagagtgtg tgtgagaggg tgtaggcatt tcataacata ttttttgtgg aataattttg
      601 gaaaaaaggt gtttttttaa taatagcttt tataagagtt tctcaaaaat tgcatgcttt
      661 ttttgaaaag gttgacaaat tatagtagta ctaaagttgc tattttcagg tctcagcaga
      721 aaattcacaa ctaaaaataa aatttgcaaa acagagtgtg cgcctttaag gtgtacagtt
      781 ttttattcct ttattgctag ttttttt
//
