LOCUS       sequence-10121-e        9380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   King GD, Muhammad AK, Xiong W, Kroeger KM, Puntel M, Larocque D, 
            Palmer D, Ng P, Lowenstein PR, Castro MG
  TITLE     High-capacity adenovirus vector-mediated anti-glioma gene therapy in
            the presence of systemic antiadenovirus immunity.
  JOURNAL   J Virol. 2008 May . 82(9):4680-4.
  PUBMED    18287240
REFERENCE   2  (bases 1 to 9380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2008 May . 82(9):4680-4."
COMMENT     Sequence Label: pAL120-TK
FEATURES             Location/Qualifiers
     source          1..9380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     promoter        1331..1866
                     /label=mCMV promoter
                     /note="mouse cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             1914..3044
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    3058..3192
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3095..3114)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3149..3168
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             6932..7123
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(7117..7139)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7225..7365
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7280..7299
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7380..7397)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7551..8139)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7631..7650)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8310..9101,9102..9170))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8933..8952
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9171..9275)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9343..9361
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtanacng gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccnag taanatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataattnt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg
      361 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc
      421 cgggtcaaag ttggcgtttt attattatag tcagctctag actcnagaag cttgaattcg
      481 agctcgccca actccgcccg ttttatgact agaaccaata gtttttaatg ccaaatgcac
      541 tgaaatcccc taatttgcaa agccaaacgc cccctatgtg agtaatacgg ggacttttta
      601 cccaatttcc caagcggaaa gccccctaat acactcatat ggcatatgaa tcagcacggt
      661 catgcactct aatggcggcc catagggact ttccacatag ggggcgttca ccatttccca
      721 gcataggggt ggtgactcaa tggcctttac ccaagtacat tgggtcaatg ggaggtaagc
      781 caatgggttt ttcccattac tggcaagcac actgagtcaa atgggacttt ccactgggtt
      841 ttgcccaagt acattgggtc aatgggaggt gagccaatgg gaaaaaccca ttgctgccaa
      901 gtacactgac tcaataggga ctttccaatg ggtttttcca ttgttggcaa gcatataagg
      961 tcaatgtggg tgagtcaata gggactttcc attgtattct gcccagtaca taaggtcaat
     1021 agggggtgaa tcaacaggaa agtcccattg gagccaagta cactgcgtca atagggactt
     1081 tccattgggt tttgcccagt acataaggtc aataggggat gagtcaatgg gaaaaaccca
     1141 ttggagccaa gtacactgac tcaataggga ctttccattg ggttttgccc agtacataag
     1201 gtcaataggg ggtgagtcaa caggaaagtc ccattggagc caagtacatt gagtcaatag
     1261 ggactttcca atgggttttg cccagtacat aaggtcaatg ggaggtaagc caatgggttt
     1321 ttcccattac tggcacgtat actgagtcat tagggacttt ccaatgggtt ttgcccagta
     1381 cataaggtca ataggggtga atcaacagga aagtcccatt ggagccaagt acactgagtc
     1441 aatagggact ttccattggg ttttgcccag tacaaaaggt caataggggg tgagtcaatg
     1501 ggtttttccc attattggca cgtacataag gtcaataggg gtgagtcatt gggtttttcc
     1561 agccaattta attaaaacgc catgtacttt cccaccattg acgtcaatgg gctattgaaa
     1621 ctaatgcaac gtgaccttta aacggtactt tcccatagct gattaatggg aaagtaccgt
     1681 tctcgagcca atacacgtca atgggaagtg aaagggcagc caaaacgtaa caccgccccg
     1741 gttttcccct ggaaattcca tattggcacg cattctattg gctgagctgc gttctacgtg
     1801 ggtataagag gcgcgaccag cgtcggtacc gtcgcagtct tcggtctgac caccgtagaa
     1861 cgcagagctc ctcgctgcag cccgggtcta gaggatccgt cgagatcccg accatggctt
     1921 cgtacccctg ccatcaacac gcgtctgcgt tcgaccaggc tgcgcgttct cgcggccata
     1981 gcaaccgacg tacggcgttg cgccctcgcc ggcagcaaga agccacggaa gtccgcctgg
     2041 agcagaaaat gcccacgcta ctgcgggttt atatagacgg tcctcacggg atggggaaaa
     2101 ccaccaccac gcaactgctg gtggccctgg gttcgcgcga cgatatcgtc tacgtacccg
     2161 agccgatgac ttactggcag gtgctggggg cttccgagac aatcgcgaac atctacacca
     2221 cacaacaccg cctcgaccag ggtgagatat cggccgggga cgcggcggtg gtaatgacaa
     2281 gcgcccagat aacaatgggc atgccttatg ccgtgaccga cgccgttctg gctcctcata
     2341 tcggggggga ggctgggagc tcacatgccc cgcccccggc cctcaccctc atcttcgacc
     2401 gccatcccat cgccgccctc ctgtgctacc cggccgcgcg ataccttatg ggcagcatga
     2461 ccccccaggc cgtgctggcg ttcgtggccc tcatcccgcc gaccttgccc ggcacaaaca
     2521 tcgtgttggg ggcccttccg gaggacagac acatcgaccg cctggccaaa cgccagcgcc
     2581 ccggcgagcg gcttgacctg gctatgctgg ccgcgattcg ccgcgtttac gggctgcttg
     2641 ccaatacggt gcggtatctg cagggcggcg ggtcgtggcg ggaggattgg ggacagcttt
     2701 cggggacggc cgtgccgccc cagggtgccg agccccagag caacgcgggc ccacgacccc
     2761 atatcgggga cacgttattt accctgtttc gggcccccga gttgctggcc cccaacggcg
     2821 acctgtacaa cgtgtttgcc tgggccttgg acgtcttggc caaacgcctc cgtcccatgc
     2881 acgtctttat cctggattac gaccaatcgc ccgccggctg ccgggacgcc ctgctgcaac
     2941 ttacctccgg gatggtccag acccacgtca ccacccccgg ctccataccg acgatctgcg
     3001 acctggcgcg cacgtttgcc cgggagatgg gggaggctaa ctgaggatct cgacgttaac
     3061 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3121 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3181 catgtctgga tccaagcttc tcgagtctag agtggaaggt gctgaggtac gatgagaccc
     3241 gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc
     3301 tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt
     3361 ttggctctag cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg
     3421 tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg
     3481 ccgccgccat gagcaccaac tcgtttgatg gaagcattgt gagctcatat ttgacaacgc
     3541 gcatgccccc atgggccggg gtgcgtcaga atgtgatggg ctccagcatt gatggtcgcc
     3601 ccgtcctgcc cgcaaactct actaccttga cctacgagac cgtgtctgga acgccgttgg
     3661 agactgcagc ctccgccgcc gcttcagccg ctgcagccac cgcccgcggg attgtgactg
     3721 actttgcttt cctgagcccg cttgcaagca gtgcagcttc ccgttcatcc gcccgcgatg
     3781 acaagttgac ggctcttttg gcacaattgg attctttgac ccgggaactt aatgtcgttt
     3841 ctcagcagct gttggatctg cgccagcagg tttctgccct gaaggcttcc tcccctccca
     3901 atgcggttta aaacataaat aaaaaaccag actctgtttg gatttggatc aagcaagtgt
     3961 cttgctgtct ttatttaggg gttttgcgcg cgcggtaggc ccgggaccag cggtctcggt
     4021 cgttgagggt cctgtgtatt ttttccagga cgtggtaaag gtgactctgg atgttcagat
     4081 acatgggcat aagcccgtct ctggggtgga ggtagcacca ctgcagagct tcatgctgcg
     4141 gggtggtgtt gtagatgatc cagtcgtagc aggagcgctg ggcgtggtgc ctaaaaatgt
     4201 ctttcagtag caagctgatt gccaggggca ggcccttggt gtaagtgttt acaaagcggt
     4261 taagctggga tgggtgcata cgtggggata tgagatgcat cttggactgt atttttaggt
     4321 tggctatgtt cccagccata tccctccggg gattcatgtt gtgcagaacc accagcacag
     4381 tgtatccggt gcacttggga aatttgtcat gtagcttaga aggaaatgcg tggaagaact
     4441 tggagacgcc cttgtgacct ccaagatttt ccatgcattc gtccataatg atggcaatgg
     4501 gcccacgggc ggcggcctgg gcgaagatat ttctgggatc actaacgtca tagttgtgtt
     4561 ccaggatgag atcgtcatag gccattttta caaagcgcgg gcggagggtg ccagactgcg
     4621 gtataatggt tccatccggc ccaggggcgt agttaccctc acagatttgc atttcccacg
     4681 ctttgagttc agatgggggg atcatgtcta cctgcggggc gatgaagaaa acggtttccg
     4741 gggtagggga gatcagctgg gaagaaagca ggttcctgag cagctgcgac ttaccgcagc
     4801 cggtgggccc gtaaatcaca cctattaccg gctgcaactg gtagttaaga gagctgcagc
     4861 tgccgtcatc cctgagcagg ggggccactt cgttaagcat gtccctgact cgcatgtttt
     4921 ccctgaccaa atccgccaga aggcgctcgc cgcccagcga tagcagttct tgcaaggaag
     4981 caaagttttt caacggtttg agaccgtccg ccgtaggcat gcttttgagc gtttgaccaa
     5041 gcagttccag gcggtcccac agctcggtca cctgctctac ggcatctcga tccagcatat
     5101 ctcctcgttt cgcgggttgg ggcggctttc gctgtacggc agtagtcggt gctcgtccag
     5161 acgggccagg gtcatgtctt tccacgggcg cagggtcctc gtcagcgtag tctgggtcac
     5221 ggtgaagggg tgcgctccgg gctgcgcgct ggccagggtg cgcttgaggc tggtcctgct
     5281 ggtgctgaag cgctgccggt cttcgccctg cgcgtcggcc aggtagcatt tgaccatggt
     5341 gtcatagtcc agcccctccg cggcgtggcc cttggcgcgc agcttgccct tggaggaggc
     5401 gccgcacgag gggcagtgca gacttttgag ggcgtagagc ttgggcgcga gaaataccga
     5461 ttccggggag taggcatccg cgccgcaggc cccgcagacg gtctcgcatt ccacgagcca
     5521 ggtgagctct ggccgttcgg ggtcaaaaac caggtttccc ccatgctttt tgatgcgttt
     5581 cttacctctg gtttccatga gccggtgtcc acgctcggtg acgaaaaggc tgtccgtgtc
     5641 cccgtataca gacttgagag gcctgtcctc gaccgatgcc cttgagagcc ttcaacccag
     5701 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
     5761 ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc
     5821 gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg
     5881 ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca
     5941 ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag
     6001 gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt
     6061 tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc
     6121 tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg gcgatttatg
     6181 ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg
     6241 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag
     6301 ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg
     6361 gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc
     6421 cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg tgcgcatgat
     6481 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa
     6541 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg
     6601 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc
     6661 agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg
     6721 aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc
     6781 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc
     6841 atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat
     6901 gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa
     6961 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga
     7021 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg
     7081 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     7141 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     7201 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     7261 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     7321 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     7381 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7441 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7501 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7561 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7621 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7681 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7741 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7801 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7861 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7921 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7981 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8041 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8101 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8161 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8221 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8281 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8341 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8401 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8461 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8521 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8581 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg
     8641 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8701 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8761 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8821 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8881 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     8941 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9001 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9061 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9121 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9181 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9241 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9301 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     9361 ccctttcgtc ttcaagaatt
//
