LOCUS       sequence-101214-        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lenti vector encoding the MS2-P65-HSF1 activator helper complex with
            a 2A Hygro resistance marker NOTE: A version of this plasmid with 
            improved titer is available: Addgene plasmid #89308 lentiMPH v2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena 
            C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F
  TITLE     Genome-scale transcriptional activation by an engineered CRISPR-Cas9
            complex.
  JOURNAL   Nature. 2014 Dec 10. doi: 10.1038/nature14136.
  PUBMED    25494202
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Dec 10. doi: 10.1038/nature14136."
COMMENT     Sequence Label: lenti MS2-P65-HSF1_Hygro
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             41..427
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             482..502
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             518..1060
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
ORIGIN
        1 ttttcttcca tttcaggtgt cgtgacgtac ggccaccatg gcttcaaact ttactcagtt
       61 cgtgctcgtg gacaatggtg ggacagggga tgtgacagtg gctccttcta atttcgctaa
      121 tggggtggca gagtggatca gctccaactc acggagccag gcctacaagg tgacatgcag
      181 cgtcaggcag tctagtgccc agaagagaaa gtataccatc aaggtggagg tccccaaagt
      241 ggctacccag acagtgggcg gagtcgaact gcctgtcgcc gcttggaggt cctacctgaa
      301 catggagctc actatcccaa ttttcgctac caattctgac tgtgaactca tcgtgaaggc
      361 aatgcagggg ctcctcaaag acggtaatcc tatcccttcc gccatcgccg ctaactcagg
      421 tatctacagc gctggaggag gtggaagcgg aggaggagga agcggaggag gaggtagcgg
      481 acctaagaaa aagaggaagg tggcggccgc tggatcccct tcagggcaga tcagcaacca
      541 ggccctggct ctggccccta gctccgctcc agtgctggcc cagactatgg tgccctctag
      601 tgctatggtg cctctggccc agccacctgc tccagcccct gtgctgaccc caggaccacc
      661 ccagtcactg agcgctccag tgcccaagtc tacacaggcc ggcgagggga ctctgagtga
      721 agctctgctg cacctgcagt tcgacgctga tgaggacctg ggagctctgc tggggaacag
      781 caccgatccc ggagtgttca cagatctggc ctccgtggac aactctgagt ttcagcagct
      841 gctgaatcag ggcgtgtcca tgtctcatag tacagccgaa ccaatgctga tggagtaccc
      901 cgaagccatt acccggctgg tgaccggcag ccagcggccc cccgaccccg ctccaactcc
      961 cctgggaacc agcggcctgc ctaatgggct gtccggagat gaagacttct caagcatcgc
     1021 tgatatggac tttagtgccc tgctgtcaca gatttcctct agtgggcagg gagg
//
