LOCUS       sequence-101216-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lenti vector encoding the MS2-P65-HSF1 activator helper complex with
            a 2A Hygro resistance marker NOTE: A version of this plasmid with 
            improved titer is available: Addgene plasmid #89308 lentiMPH v2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena 
            C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F
  TITLE     Genome-scale transcriptional activation by an engineered CRISPR-Cas9
            complex.
  JOURNAL   Nature. 2014 Dec 10. doi: 10.1038/nature14136.
  PUBMED    25494202
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Dec 10. doi: 10.1038/nature14136."
COMMENT     Sequence Label: lenti MS2-P65-HSF1_Hygro
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(109..162)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(178..549)
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
ORIGIN
        1 ccgagattct tcgccctcgg agagctgcat caggtcggag acgctgtcga acttttcgat
       61 cagaaacttc tcgacagagg tagcggtgag ttcaggcttt ttcatggttg ggccaggatt
      121 ctcctcgacg tcaccgcatg ttagcagact tcctctgccc tctccactgc ctgtacagga
      181 gacagtgggg tccttggctt tgggaggctc cgagcctgtc agcagggaga tggtggggtc
      241 ctcggcgaag ccgtcccctt cggagaagta ggagccctct cccagctcaa acagcaccgg
      301 caggtcgttg ctcccggtgt ccacggagcc ggggtccagc aggaacagcg gctgcgctgt
      361 gtagtgcacc agctgcttcc ctgaatccgg gctgctgttc tctgcctcgg gaggcctggg
      421 gggctcctgg ggagacagga gctcttggat actggccagg ctgctgtcaa ggtcaggcag
      481 gctcatgtcg ggcacggtca ccgaggggct gaacaggtcc agcagggcac tggtgtccac
      541 gctgaagccg cttccacctc ctccctgccc actagaggaa atctgtgaca gcagggcact
      601 aaagtccata tcagcgatgc ttgagaagtc ttcatctccg gacagcccat taggcaggcc
      661 gctggttccc aggggagttg gagcggggtc ggggggccgc tggctgccgg tcaccagccg
      721 ggtaatggct tcggggtact ccatcagcat tggttcggct gtactatgag acatggacac
      781 gccctgattc agcagctgct gaaactcaga gttgtccacg gaggccagat ctgtgaacac
      841 tccgggatcg gtgctgttcc ccagcagagc tcccaggtcc tcatcagcgt cgaactgcag
      901 gtgcagcaga gcttcactca gagtcccctc gccggcctgt gtagacttgg g
//
