LOCUS       sequence-10122-e        8230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   King GD, Muhammad AK, Xiong W, Kroeger KM, Puntel M, Larocque D, 
            Palmer D, Ng P, Lowenstein PR, Castro MG
  TITLE     High-capacity adenovirus vector-mediated anti-glioma gene therapy in
            the presence of systemic antiadenovirus immunity.
  JOURNAL   J Virol. 2008 May . 82(9):4680-4.
  PUBMED    18287240
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2008 May . 82(9):4680-4."
COMMENT     Sequence Label: pAL120
FEATURES             Location/Qualifiers
     source          1..8230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     promoter        1331..1866
                     /label=mCMV promoter
                     /note="mouse cytomegalovirus (CMV) immediate early 
                     promoter"
     polyA_signal    1908..2042
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1945..1964)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1999..2018
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             5782..5973
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(5967..5989)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6075..6215
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6130..6149
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6230..6247)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6401..6989)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6481..6500)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7160..7951,7952..8020))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7783..7802
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8021..8125)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8193..8211
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtanacng gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccnag taanatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataattnt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg
      361 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc
      421 cgggtcaaag ttggcgtttt attattatag tcagctctag actcnagaag cttgaattcg
      481 agctcgccca actccgcccg ttttatgact agaaccaata gtttttaatg ccaaatgcac
      541 tgaaatcccc taatttgcaa agccaaacgc cccctatgtg agtaatacgg ggacttttta
      601 cccaatttcc caagcggaaa gccccctaat acactcatat ggcatatgaa tcagcacggt
      661 catgcactct aatggcggcc catagggact ttccacatag ggggcgttca ccatttccca
      721 gcataggggt ggtgactcaa tggcctttac ccaagtacat tgggtcaatg ggaggtaagc
      781 caatgggttt ttcccattac tggcaagcac actgagtcaa atgggacttt ccactgggtt
      841 ttgcccaagt acattgggtc aatgggaggt gagccaatgg gaaaaaccca ttgctgccaa
      901 gtacactgac tcaataggga ctttccaatg ggtttttcca ttgttggcaa gcatataagg
      961 tcaatgtggg tgagtcaata gggactttcc attgtattct gcccagtaca taaggtcaat
     1021 agggggtgaa tcaacaggaa agtcccattg gagccaagta cactgcgtca atagggactt
     1081 tccattgggt tttgcccagt acataaggtc aataggggat gagtcaatgg gaaaaaccca
     1141 ttggagccaa gtacactgac tcaataggga ctttccattg ggttttgccc agtacataag
     1201 gtcaataggg ggtgagtcaa caggaaagtc ccattggagc caagtacatt gagtcaatag
     1261 ggactttcca atgggttttg cccagtacat aaggtcaatg ggaggtaagc caatgggttt
     1321 ttcccattac tggcacgtat actgagtcat tagggacttt ccaatgggtt ttgcccagta
     1381 cataaggtca ataggggtga atcaacagga aagtcccatt ggagccaagt acactgagtc
     1441 aatagggact ttccattggg ttttgcccag tacaaaaggt caataggggg tgagtcaatg
     1501 ggtttttccc attattggca cgtacataag gtcaataggg gtgagtcatt gggtttttcc
     1561 agccaattta attaaaacgc catgtacttt cccaccattg acgtcaatgg gctattgaaa
     1621 ctaatgcaac gtgaccttta aacggtactt tcccatagct gattaatggg aaagtaccgt
     1681 tctcgagcca atacacgtca atgggaagtg aaagggcagc caaaacgtaa caccgccccg
     1741 gttttcccct ggaaattcca tattggcacg cattctattg gctgagctgc gttctacgtg
     1801 ggtataagag gcgcgaccag cgtcggtacc gtcgcagtct tcggtctgac caccgtagaa
     1861 cgcagagctc ctcgctgcag cccgggtcta gaggatccgt cgacgttaac ttgtttattg
     1921 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1981 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     2041 tccaagcttc tcgagtctag agtggaaggt gctgaggtac gatgagaccc gcaccaggtg
     2101 cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc tggatgtgac
     2161 cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt ttggctctag
     2221 cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg tgggaaagaa
     2281 tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg ccgccgccat
     2341 gagcaccaac tcgtttgatg gaagcattgt gagctcatat ttgacaacgc gcatgccccc
     2401 atgggccggg gtgcgtcaga atgtgatggg ctccagcatt gatggtcgcc ccgtcctgcc
     2461 cgcaaactct actaccttga cctacgagac cgtgtctgga acgccgttgg agactgcagc
     2521 ctccgccgcc gcttcagccg ctgcagccac cgcccgcggg attgtgactg actttgcttt
     2581 cctgagcccg cttgcaagca gtgcagcttc ccgttcatcc gcccgcgatg acaagttgac
     2641 ggctcttttg gcacaattgg attctttgac ccgggaactt aatgtcgttt ctcagcagct
     2701 gttggatctg cgccagcagg tttctgccct gaaggcttcc tcccctccca atgcggttta
     2761 aaacataaat aaaaaaccag actctgtttg gatttggatc aagcaagtgt cttgctgtct
     2821 ttatttaggg gttttgcgcg cgcggtaggc ccgggaccag cggtctcggt cgttgagggt
     2881 cctgtgtatt ttttccagga cgtggtaaag gtgactctgg atgttcagat acatgggcat
     2941 aagcccgtct ctggggtgga ggtagcacca ctgcagagct tcatgctgcg gggtggtgtt
     3001 gtagatgatc cagtcgtagc aggagcgctg ggcgtggtgc ctaaaaatgt ctttcagtag
     3061 caagctgatt gccaggggca ggcccttggt gtaagtgttt acaaagcggt taagctggga
     3121 tgggtgcata cgtggggata tgagatgcat cttggactgt atttttaggt tggctatgtt
     3181 cccagccata tccctccggg gattcatgtt gtgcagaacc accagcacag tgtatccggt
     3241 gcacttggga aatttgtcat gtagcttaga aggaaatgcg tggaagaact tggagacgcc
     3301 cttgtgacct ccaagatttt ccatgcattc gtccataatg atggcaatgg gcccacgggc
     3361 ggcggcctgg gcgaagatat ttctgggatc actaacgtca tagttgtgtt ccaggatgag
     3421 atcgtcatag gccattttta caaagcgcgg gcggagggtg ccagactgcg gtataatggt
     3481 tccatccggc ccaggggcgt agttaccctc acagatttgc atttcccacg ctttgagttc
     3541 agatgggggg atcatgtcta cctgcggggc gatgaagaaa acggtttccg gggtagggga
     3601 gatcagctgg gaagaaagca ggttcctgag cagctgcgac ttaccgcagc cggtgggccc
     3661 gtaaatcaca cctattaccg gctgcaactg gtagttaaga gagctgcagc tgccgtcatc
     3721 cctgagcagg ggggccactt cgttaagcat gtccctgact cgcatgtttt ccctgaccaa
     3781 atccgccaga aggcgctcgc cgcccagcga tagcagttct tgcaaggaag caaagttttt
     3841 caacggtttg agaccgtccg ccgtaggcat gcttttgagc gtttgaccaa gcagttccag
     3901 gcggtcccac agctcggtca cctgctctac ggcatctcga tccagcatat ctcctcgttt
     3961 cgcgggttgg ggcggctttc gctgtacggc agtagtcggt gctcgtccag acgggccagg
     4021 gtcatgtctt tccacgggcg cagggtcctc gtcagcgtag tctgggtcac ggtgaagggg
     4081 tgcgctccgg gctgcgcgct ggccagggtg cgcttgaggc tggtcctgct ggtgctgaag
     4141 cgctgccggt cttcgccctg cgcgtcggcc aggtagcatt tgaccatggt gtcatagtcc
     4201 agcccctccg cggcgtggcc cttggcgcgc agcttgccct tggaggaggc gccgcacgag
     4261 gggcagtgca gacttttgag ggcgtagagc ttgggcgcga gaaataccga ttccggggag
     4321 taggcatccg cgccgcaggc cccgcagacg gtctcgcatt ccacgagcca ggtgagctct
     4381 ggccgttcgg ggtcaaaaac caggtttccc ccatgctttt tgatgcgttt cttacctctg
     4441 gtttccatga gccggtgtcc acgctcggtg acgaaaaggc tgtccgtgtc cccgtataca
     4501 gacttgagag gcctgtcctc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     4561 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     4621 actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg
     4681 gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca
     4741 agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg
     4801 catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc
     4861 cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat
     4921 gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct
     4981 taccagccta acttcgatca ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc
     5041 gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc
     5101 cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag ccggcggcac
     5161 ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg gagaactgtg
     5221 aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc cagcagccgc
     5281 acgcggcgca tctcgggcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg
     5341 tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg
     5401 atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca
     5461 tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc
     5521 accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca
     5581 tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc
     5641 cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta
     5701 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat
     5761 cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc
     5821 tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa
     5881 caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc
     5941 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     6001 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     6061 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
     6121 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     6181 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
     6241 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6301 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6361 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6421 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6481 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6541 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6601 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6661 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6721 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6781 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6841 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6901 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6961 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7021 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7081 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7141 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7201 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7261 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7321 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7381 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7441 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
     7501 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7561 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7621 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7681 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7741 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     7801 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7861 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7921 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7981 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8041 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8101 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     8161 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     8221 ttcaagaatt
//
