LOCUS       sequence-101225-         518 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a gRNA plasmid targeting the prion gene's exon 3, negative
            strand.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Mehrabian M, Brethour D, MacIsaac S, Kim JK, Gunawardana CG, Wang H,
            Schmitt-Ulms G
  TITLE     CRISPR-Cas9-Based Knockout of the Prion Protein and Its Effect on 
            the Proteome.
  JOURNAL   PLoS One. 2014 Dec 9;9(12):e114594. doi: 
            10.1371/journal.pone.0114594. eCollection 2014.
  PUBMED    25490046
REFERENCE   2  (bases 1 to 518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-12-9"; volume: "9(12):e114594. doi"; pages: " 
            10.1371/journal.pone.0114594. eCollection 2014"
COMMENT     Sequence Label: MLM3636-Prnp-CDS-Neg
FEATURES             Location/Qualifiers
     source          1..518
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        155..403
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     155..175
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     326..345
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        424..499
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tacatatttg aatgtattta gtaaaaataa acaaataggg gttccgcgca catttccccg
       61 aaaagtgcca cctgacgtcg ctagctgtac aaaaaagcag gctttaaagg aaccaattca
      121 gtcgactgga tccggtacca aggtcgggca ggaagagggc ctatttccca tgattccttc
      181 atatttgcat atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa
      241 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
      301 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
      361 cgatttcttg gctttatata tcttgtggaa aggacgaaac accttggccc catccaccgc
      421 catgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
      481 agtggcaccg agtcggtgct ttttttaanc ttgggccg
//
