LOCUS       sequence-101226-         578 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a gRNA plasmid targeting the prion gene's exon 3, positive
            strand.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Mehrabian M, Brethour D, MacIsaac S, Kim JK, Gunawardana CG, Wang H,
            Schmitt-Ulms G
  TITLE     CRISPR-Cas9-Based Knockout of the Prion Protein and Its Effect on 
            the Proteome.
  JOURNAL   PLoS One. 2014 Dec 9;9(12):e114594. doi: 
            10.1371/journal.pone.0114594. eCollection 2014.
  PUBMED    25490046
REFERENCE   2  (bases 1 to 578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-12-9"; volume: "9(12):e114594. doi"; pages: " 
            10.1371/journal.pone.0114594. eCollection 2014"
COMMENT     Sequence Label: MLM3636-Prnp-CDS-Pos
FEATURES             Location/Qualifiers
     source          1..578
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        156..404
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     156..176
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     327..346
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tncatatttt gaatgtattt agtaaaaata aacaaatagg ggttccgcgc acatttcccc
       61 gaaaagtgcc acctgacgtc gctagctgta caaaaaagca ggctttaaag gaaccaattc
      121 agtcgactgg atccggtacc aaggtcgggc aggaagaggg cctatttccc atgattcctt
      181 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa
      241 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
      301 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
      361 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggtgga acaccggtgg
      421 aagcgtttta nagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
      481 aagtggcacc gagtcngtgc ttttttnaan cttgggccgn tcgaggtacc tctctacata
      541 tgacatgtgn gcaaaaggcc ncaaaaggcn annanccg
//
