LOCUS       sequence-101228-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Ward JD
  TITLE     Rapid and Precise Engineering of the Caenorhabditis elegans Genome 
            with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair.
  JOURNAL   Genetics. 2014 Dec 9. pii: genetics.114.172361.
  PUBMED    25491644
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 Dec 9. pii: genetics.114.172361."
COMMENT     Sequence Label: pJW1219
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(331..357)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(358..378)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcttggcggc acaataaagt ttcctctttt tgttgggtca ctcaacgaac acaatacaaa
       61 tatttgagcg ctcgcatttt tctcaacctt tcgggtttaa agcacttcaa aatggaattg
      121 aagagagatt gtgaaagtac atcagacaga atcacatttt gatagcattc acttcactca
      181 gatgcaaact tttcagtgac tataaaaagt cataaaataa taacattaaa aaagcaaata
      241 aattagcgag agaatttttt gacaaaaaga aagaagagtg atagagaaga agggaatgct
      301 tgaaaggatt ttgcatttat cactagttta ggcgtagtct gggacgtcgt atgggtagac
      361 cttacgcttc ttctttggtg gtccggatcc tccgtctcct ccgagttggg agaggtcgat
      421 acgggtctcg tagagtccgg tgatggattg gtggatgagg gtggcgtcga ggacctcctt
      481 ggtggaggtg taacgcttac ggtcgatggt ggtgtcgaag tacttgaagg cggctggggc
      541 tccgaggttg gtgagggtga agaggtggat gatgttctcg gcttgctcac ggattggctt
      601 gtcacggtgc ttgttgtagg cggagaggac cttgtcgagg ttggcgtcgg cgaggatgac
      661 acgcttggag aactcggaga tttgctcgat gatctcgtcg aggtagtgct tgtgttgctc
      721 gacgaagagt tgcttttgct cgttgtcctc tggggatccc ttgagcttct cgtagtggga
      781 ggcgaggtag aggaagttga cgtacttgga tgggagggcg agctcgtttc ccttttggag
      841 ctctccg
//
