LOCUS       sequence-10123-e        8257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   King GD, Muhammad AK, Xiong W, Kroeger KM, Puntel M, Larocque D, 
            Palmer D, Ng P, Lowenstein PR, Castro MG
  TITLE     High-capacity adenovirus vector-mediated anti-glioma gene therapy in
            the presence of systemic antiadenovirus immunity.
  JOURNAL   J Virol. 2008 May . 82(9):4680-4.
  PUBMED    18287240
REFERENCE   2  (bases 1 to 8257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2008 May . 82(9):4680-4."
COMMENT     Sequence Label: pAL120-modified
FEATURES             Location/Qualifiers
     source          1..8257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     promoter        1331..1866
                     /label=mCMV promoter
                     /note="mouse cytomegalovirus (CMV) immediate early 
                     promoter"
     polyA_signal    1933..2067
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1970..1989)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2024..2043
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             5807..5998
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(5992..6014)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6100..6240
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6155..6174
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6255..6272)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6426..7014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6506..6525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7185..7976,7977..8045))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7808..7827
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8046..8150)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8218..8236
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg
      361 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc
      421 cgggtcaaag ttggcgtttt attattatag tcagctctag actcgagaag cttgaattcg
      481 agctcgccca actccgcccg ttttatgact agaaccaata gtttttaatg ccaaatgcac
      541 tgaaatcccc taatttgcaa agccaaacgc cccctatgtg agtaatacgg ggacttttta
      601 cccaatttcc caagcggaaa gccccctaat acactcatat ggcatatgaa tcagcacggt
      661 catgcactct aatggcggcc catagggact ttccacatag ggggcgttca ccatttccca
      721 gcataggggt ggtgactcaa tggcctttac ccaagtacat tgggtcaatg ggaggtaagc
      781 caatgggttt ttcccattac tggcaagcac actgagtcaa atgggacttt ccactgggtt
      841 ttgcccaagt acattgggtc aatgggaggt gagccaatgg gaaaaaccca ttgctgccaa
      901 gtacactgac tcaataggga ctttccaatg ggtttttcca ttgttggcaa gcatataagg
      961 tcaatgtggg tgagtcaata gggactttcc attgtattct gcccagtaca taaggtcaat
     1021 agggggtgaa tcaacaggaa agtcccattg gagccaagta cactgcgtca atagggactt
     1081 tccattgggt tttgcccagt acataaggtc aataggggat gagtcaatgg gaaaaaccca
     1141 ttggagccaa gtacactgac tcaataggga ctttccattg ggttttgccc agtacataag
     1201 gtcaataggg ggtgagtcaa caggaaagtc ccattggagc caagtacatt gagtcaatag
     1261 ggactttcca atgggttttg cccagtacat aaggtcaatg ggaggtaagc caatgggttt
     1321 ttcccattac tggcacgtat actgagtcat tagggacttt ccaatgggtt ttgcccagta
     1381 cataaggtca ataggggtga atcaacagga aagtcccatt ggagccaagt acactgagtc
     1441 aatagggact ttccattggg ttttgcccag tacaaaaggt caataggggg tgagtcaatg
     1501 ggtttttccc attattggca cgtacataag gtcaataggg gtgagtcatt gggtttttcc
     1561 agccaattta attaaaacgc catgtacttt cccaccattg acgtcaatgg gctattgaaa
     1621 ctaatgcaac gtgaccttta aacggtactt tcccatagct gattaatggg aaagtaccgt
     1681 tctcgagcca atacacgtca atgggaagtg aaagggcagc caaaacgtaa caccgccccg
     1741 gttttcccct ggaaattcca tattggcacg cattctattg gctgagctgc gttctacgtg
     1801 ggtataagag gcgcgaccag cgtcggtacc gtcgcagtct tcggtctgac caccgtagaa
     1861 cgcagagctc ctcgctgcag cccgggtcta gaggatccgt cgaggatatc gtcgacacta
     1921 gtcctcgacg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     1981 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     2041 atcaatgtat cttatcatgt ctggatccaa gcttctcgag tctagagtgg aaggtgctga
     2101 ggtacgatga gacccgcacc aggtgcagac cctgcgagtg tggcggtaaa catattagga
     2161 accagcctgt gatgctggat gtgaccgagg agctgaggcc cgatcacttg gtgctggcct
     2221 gcacccgcgc tgagtttggc tctagcgatg aagatacaga ttgaggtact gaaatgtgtg
     2281 ggcgtggctt aagggtggga aagaatatat aaggtggggg tcttatgtag ttttgtatct
     2341 gttttgcagc agccgccgcc gccatgagca ccaactcgtt tgatggaagc attgtgagct
     2401 catatttgac aacgcgcatg cccccatggg ccggggtgcg tcagaatgtg atgggctcca
     2461 gcattgatgg tcgccccgtc ctgcccgcaa actctactac cttgacctac gagaccgtgt
     2521 ctggaacgcc gttggagact gcagcctccg ccgccgcttc agccgctgca gccaccgccc
     2581 gcgggattgt gactgacttt gctttcctga gcccgcttgc aagcagtgca gcttcccgtt
     2641 catccgcccg cgatgacaag ttgacggctc ttttggcaca attggattct ttgacccggg
     2701 aacttaatgt cgtttctcag cagctgttgg atctgcgcca gcaggtttct gccctgaagg
     2761 cttcctcccc tcccaatgcg gtttaaaaca taaataaaaa accagactct gtttggattt
     2821 ggatcaagca agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg taggcccggg
     2881 accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg taaaggtgac
     2941 tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag caccactgca
     3001 gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag cgctgggcgt
     3061 ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc ttggtgtaag
     3121 tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga tgcatcttgg
     3181 actgtatttt taggttggct atgttcccag ccatatccct ccggggattc atgttgtgca
     3241 gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc ttagaaggaa
     3301 atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg cattcgtcca
     3361 taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg ggatcactaa
     3421 cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag cgcgggcgga
     3481 gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta ccctcacaga
     3541 tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc ggggcgatga
     3601 agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc ctgagcagct
     3661 gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccgggtgc aactggtagt
     3721 taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta agcatgtccc
     3781 tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc agcgatagca
     3841 gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta ggcatgcttt
     3901 tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc tctacggcat
     3961 ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt acggcagtag
     4021 tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg tcctcgtcag
     4081 cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca gggtgcgctt
     4141 gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt cggccaggta
     4201 gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg cgcgcagctt
     4261 gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt agagcttggg
     4321 cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc agacggtctc
     4381 gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt ttcccccatg
     4441 ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct cggtgacgaa
     4501 aaggctgtcc gtgtccccgt atacagactt gagaggcctg tcctcgaccg atgcccttga
     4561 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     4621 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
     4681 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     4741 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
     4801 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
     4861 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
     4921 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
     4981 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcactgga ccgctgatcg
     5041 tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg
     5101 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct
     5161 cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc
     5221 caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat
     5281 cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc
     5341 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct
     5401 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa
     5461 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct
     5521 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg
     5581 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt
     5641 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag
     5701 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg
     5761 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga
     5821 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa
     5881 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc
     5941 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     6001 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     6061 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     6121 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     6181 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     6241 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6301 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6361 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6421 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6481 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6541 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6601 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6661 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6721 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6781 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6841 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     6901 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6961 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7021 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7081 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7141 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7201 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     7261 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     7321 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7381 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7441 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7501 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7561 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7621 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7681 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7741 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7801 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7861 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7921 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7981 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8041 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8101 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8161 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     8221 aggcgtatca cgaggccctt tcgtcttcaa gaattcc
//
