LOCUS       sequence-101236-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 plasmid with sgRNA(F+E) targeting pha-1; for 
            co-conversion in C. elegans .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Ward JD
  TITLE     Rapid and Precise Engineering of the Caenorhabditis elegans Genome 
            with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair.
  JOURNAL   Genetics. 2014 Dec 9. pii: genetics.114.172361.
  PUBMED    25491644
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 Dec 9. pii: genetics.114.172361."
COMMENT     Sequence Label: pJW1285
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(333..359)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(360..380)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttcttggcg gcacaataaa gtttcctctt tttgttgggt cactcaacga acacaataca
       61 aatatttgag cgctcgcatt tttctcaacc tttcgggttt aaagcacttc aaaatggaat
      121 tgaagagaga ttgtgaaagt acatcagaca gaatcacatt ttgatagcat tcacttcact
      181 cagatgcaaa cttttcagtg actataaaaa gtcataaaat aataacatta aaaaagcaaa
      241 taaattagcg agagaatttt ttgacaaaaa gaaagaagag tgatagagaa gaagggaatg
      301 cttgaaagga ttttgcattt atcactagtt taggcgtagt ctgggacgtc gtatgggtag
      361 accttacgct tcttctttgg tggtccggat cctccgtctc ctccgagttg ggagaggtcg
      421 atacgggtct cgtagagtcc ggtgatggat tggtggatga gggtggcgtc gaggacctcc
      481 ttggtggagg tgtaacgctt acggtcgatg gtggtgtcga agtacttgaa ggcggctggg
      541 gctccgaggt tggtgagggt gaagaggtgg atgatgttct cggcttgctc acggattggc
      601 ttgtcacggt gcttgttgta ggcggagagg accttgtcga ggttggcgtc ggcgaggatg
      661 acacgcttgg agaactcgga gatttgctcg atgatctcgt cgaggtagtg cttgtgttgc
      721 tcgacgaaga gttgcttttg ctcgttgtcc tctggggatc ccttgagctt ctcgtagtgg
      781 gaggcgaggt agaggaagtt gacgtacttg gatgggaggg cgagctcgtt tcccttttgg
      841 agctctccgg cggaggcgag catacgctta cgtccgttct cgagctcgaa
//
