LOCUS       sequence-101256-        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent Cas9 expression plasmid and targeting vector for the 
            mouse Rosa26 locus. Cas9 expression is Cre-dependent and the 
            targeting vector has positive (Neo) and negative (DTA) selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014."
COMMENT     Sequence Label: LSL-Cas9-Rosa26TV
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..33
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15..33
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    95..319
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     308..325
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     intron          393..458
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctgggtac cgggcccccc
       61 ctcgagcccc agctggttct ttccgcctca gaagccatag agcccaccgc atccccagca
      121 tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga
      181 atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca
      241 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
      301 tggcaactag aaggcacagt cgaggctgat cagcgagctc taggatctgc attccaccac
      361 tgctcccatt catcagttcc ataggttgga atctaaaata cacaaacaat tagaatcagt
      421 agtttaacac attatacact taaaaatttt atatttacct tagagcttta aatctctgta
      481 ggtagtttgt ccaattatgt cacaccacag aagtaaggtt ccttcacaaa gagatcgcct
      541 gacacgattt cctgcacagg cttgagccat atactcatac atcgcatctt ggccacgttt
      601 tccacgggtt tcaaaattaa tctcaagttc tacgcttaac gctttcgcct gttcccagtt
      661 attaatatat tcaacgctag aactcccctc agcgaaggga aggctgagca ctacacgcga
      721 agcaccatca ccgaaccttt tgataaactc ttccgttccg acttgctcca tcaacggttc
      781 agtgagactt aaacctaact ctttcttaat agtttcggca ttatccactt ttagtgcgag
      841 aaccttcgtc agtcctggat acgtcacttt gaccacgcct ccagcttttc cagagagcgg
      901 gttttcatta tctacagagt atcccgcagc gtcgtattta ttgtcggtac tataaaaccc
      961 tttccaatca tcgtcataat ttccttgtgt accagatttt ggcttttgta tacctttttg
     1021 aatg
//
