LOCUS       sequence-101278-         558 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LINuS-flex: modular LINuS construct enabling introduction of any 
            protein encoding sequence, NES and NLS. The sequence of interest 
            replaces the LexA DBD-MBP-VP64 fragment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Niopek D, Benzinger D, Roensch J, Draebing T, Wehler P, Eils R, Di 
            Ventura B
  TITLE     Engineering light-inducible nuclear localization signals for precise
            spatiotemporal control of protein dynamics in living cells.
  JOURNAL   Nat Commun. 2014 Jul 14;5:4404. doi: 10.1038/ncomms5404.
  PUBMED    25019686
REFERENCE   2  (bases 1 to 558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-07-14"; volume: "5:4404. doi"; pages: " 
            10.1038/ncomms5404"
COMMENT     Sequence Label: pDN80
FEATURES             Location/Qualifiers
     source          1..558
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(175..285)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     primer_bind     complement(334..357)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     regulatory      465..474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc
       61 catgtgcacc ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt
      121 gctcactgag acgacgctgc cgccgctgcc gccggcgtct ccactagtcc cggggagcat
      181 gtcaaggtca aaatcgtcaa gagcgtcagc aggcagcata tcaaggtcaa agtcgtcaag
      241 ggcatcggct gggagcatgt ctaagtcaaa atcgtcaagg gcgtcggccg gcccgccgct
      301 ttcggtaccg ctgccgccct tggtgatacg agtctgcgcg tctttcaggg cttcatcgac
      361 agtctgacga ccgctggcgg cgttgatcac cgcagtacgc acggcatacc agaaagcgga
      421 catctgcggg atgttcggca tgatttcacc tttctgggcg ttttccatgg tggcggcaat
      481 acgtggatct ttcgccaact cttcctcgta agacttcagc gctacggcac ccagcggttt
      541 gtctttatta accgcttc
//
