LOCUS       sequence-101279-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LINuS-flex: modular LINuS construct enabling introduction of any 
            protein encoding sequence, NES and NLS. The sequence of interest 
            replaces the LexA DBD-MBP-VP64 fragment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Niopek D, Benzinger D, Roensch J, Draebing T, Wehler P, Eils R, Di 
            Ventura B
  TITLE     Engineering light-inducible nuclear localization signals for precise
            spatiotemporal control of protein dynamics in living cells.
  JOURNAL   Nat Commun. 2014 Jul 14;5:4404. doi: 10.1038/ncomms5404.
  PUBMED    25019686
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-07-14"; volume: "5:4404. doi"; pages: " 
            10.1038/ncomms5404"
COMMENT     Sequence Label: pDN80
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(885..904)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatct agagtcgcgg ccgctgagac ctttaatttc tactattggt ctccaatatt
      181 ttctgcagtt ttcttaatca gcatgactcc ctctctctcg gcagcatctc ggacatgctc
      241 agttccatcc aactgaaccc caataaagta ctggacatct cccttctgat ctcgcatagg
      301 ctgcaagtga aagaggttcc agaacttttt accactcttt gtataattaa tcagctgaac
      361 agtgacctct gtttggttat ctatggcatc tctaattttt ctcactgtcg cgcgatcagt
      421 ttcaggacct tgtagaaacc tgcagtttct tcccaaaatt tcttcacggc tatattctgt
      481 caactgcaag aaactatcgg acgcgaatat aatgggatta tctggcaatc ttgggtcagt
      541 aatgacaaag ttcttctcaa tacgttcaag tgtagtagcc aaacttccac ctgaacctcc
      601 agatccaccc ttgtacagct cgtccatgcc gccggtggag tggcggccct cggcgcgttc
      661 gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg atgtccaact tgatgttgac
      721 gttgtaggcg ccgggcagct gcacgggctt cttggccttg taggtggtct tgacctcagc
      781 gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg atctcgccct tcagggcgcc
      841 gtcctcgggg tacatccgct cggaggaggc ctcccagccc atggtcttct tctgcattac
      901 ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc ttgtagatga actcgccgtc
      961 ctgcagggag gagtcctgg
//
