LOCUS       sequence-101280-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LINuS-flex: modular LINuS construct enabling introduction of any 
            protein encoding sequence, NES and NLS. The sequence of interest 
            replaces the LexA DBD-MBP-VP64 fragment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Niopek D, Benzinger D, Roensch J, Draebing T, Wehler P, Eils R, Di 
            Ventura B
  TITLE     Engineering light-inducible nuclear localization signals for precise
            spatiotemporal control of protein dynamics in living cells.
  JOURNAL   Nat Commun. 2014 Jul 14;5:4404. doi: 10.1038/ncomms5404.
  PUBMED    25019686
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-07-14"; volume: "5:4404. doi"; pages: " 
            10.1038/ncomms5404"
COMMENT     Sequence Label: pDN80
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    39..119
                     /label=MCS
                     /note="multiple cloning site"
     CDS             127..378
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggtc
      121 gccaccatga aagcgttaac ggccaggcaa caagaggtgt ttgatctcat ccgtgatcac
      181 atcagccaga caggtatgcc gccgacgcgt gcggaaatcg cgcagcgttt ggggttccgt
      241 tccccaaacg cggctgaaga acatctgaag gcgctggcac gcaaaggcgt tattgaaatt
      301 gtttccggcg catcacgcgg gattcgtctg ttgcaggaag aggaagaagg gttgccgctg
      361 gtaggtcgtg tggctgccgg tgaaccaggt ggtagcggaa ccagcaaaat aaaaacaggt
      421 gcacgcatcc tcgcattatc cgcattaacg acgatgatgt tttccgcctc ggctctcgcc
      481 aaaatcgaag aaggtaaact ggtaatctgg attaacggcg ataaaggcta taacggcctc
      541 gctgaagtcg gtaagaaatt cgagaaagat accggaatta aagtcaccgt tgagcatccg
      601 gataaactgg aagagaaatt cccacaggtt gcggcaactg gcgatggccc tgacattatc
      661 ttctgggcac acgaccgctt tggtggctac gctcaatctg gcctgttggc tgaaatcacc
      721 ccggacaaag cgttccagga caagctgtat ccgtttacct gggatgccgt acgttacaac
      781 ggcaagctga ttgcttaccc gatcgctgtt gaagcgttat cgctgattta taacaaagat
      841 ctgctgccga acccgccaaa aacctgggaa gagatcccgg cgctggataa agaactgaaa
      901 gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac cgtacttcac ctggccgctg
      961 attgctgctg acgggggtta tgcgttcaag tatgaaaacg gcaagtacga cattaaagac
     1021 gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa
//
