LOCUS       sequence-10129-e        3033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Gully D, Bouveret E
  TITLE     A protein network for phospholipid synthesis uncovered by a variant 
            of the tandem affinity purification method in Escherichia coli.
  JOURNAL   Proteomics. 2006 Jan . 6(1):282-93.
  PUBMED    16294310
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteomics.
            2006 Jan . 6(1):282-93."
COMMENT     Sequence Label: pEB355
FEATURES             Location/Qualifiers
     source          1..3033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(588..604)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(589..605)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(647..666)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     766..788
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(826..844)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        912..1016
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1017..1085,1086..1877)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1235..1254)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2048..2636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2537..2556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2790..2807
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2924..2945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2960..2965,2966..2983,2984..2990)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2998..3014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3003..3025
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(3022..3033,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3022..3033,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttagccgcc agcgaggcca cgggcggcct ggatcgcgaa
       61 cgcatcgact tgttgtggaa aatcgctcgc gccggcgccc gttccgcagt gggcaccgag
      121 gcgcgtcgcc agttccgcta cgacggcgac atgaatatcg gcgtgatcac cgatttcgag
      181 ctggaagtgc gcaatgcgct gaacaggcgg gcgcacgccg tcggcgcgca ggacgtggtc
      241 cagcatggca ctgagcagaa caatcctttc ccggaggcag atgagaagat tttcgtcgta
      301 tcggccaccg gtgaaagcca gatgctcacg cgcgggcaac tgaaggaata cattggccag
      361 cagcgcggcg agggctatgt cttctacgag aaccgtgcat acggcgtggc ggggaaaagc
      421 ctgttcgacg atgggctggg agccgcgccc ggcgtgccga gcggacgttc gaagttctcg
      481 ccggatgtac tggaaacggt gccggcgtca cccggattgc ggcggccgtc gctgggcgca
      541 gtggaacgcc actgcaggtc gactctagaa ttcgatatca agcttatcga taccgtcgac
      601 ctcgagcaat tcatcgatat aactaagtaa tatggtgcac tctcagtaca atctgctctg
      661 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
      721 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
      781 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
      841 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      901 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      961 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     1021 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     1081 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     1141 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     1201 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     1261 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     1321 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1381 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     1441 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1501 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1561 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1621 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1681 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1741 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1801 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1861 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1921 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1981 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2041 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2101 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2161 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     2221 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2281 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2341 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2401 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2461 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2521 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2581 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2641 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2701 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2761 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     2821 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     2881 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     2941 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     3001 tgagcggata acaatttcac acaggaaaca gct
//
