LOCUS       sequence-101292-         503 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by 
            PCR-fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Ward JD
  TITLE     Rapid and Precise Engineering of the Caenorhabditis elegans Genome 
            with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair.
  JOURNAL   Genetics. 2014 Dec 9. pii: genetics.114.172361.
  PUBMED    25491644
REFERENCE   2  (bases 1 to 503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 Dec 9. pii: genetics.114.172361."
COMMENT     Sequence Label: pJW1311
FEATURES             Location/Qualifiers
     source          1..503
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(334..353)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(335..353)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(360..377)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(360..376)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(369..391)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
       61 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
      121 aaatttcaca aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc
      181 cttatttaaa cttgctatgc tgtttccagc atagctctta aactcagggt ttcagcctcg
      241 ttcaagacat ctcgcaatag gaggaagggc gaattctgca gatatccatc acactggcgg
      301 ccgctcgagc atgcatctag agggcccaat tcgccctata gtgagtcgta ttacaattca
      361 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      421 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      481 ccttcccaac agttgcgcag cct
//
