LOCUS       sequence-101299-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luminescent reporter of insulin secretion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Burns SM, Vetere A, Walpita D, Dancik V, Khodier C, Perez J, Clemons
            PA, Wagner BK, Altshuler D
  TITLE     High-throughput luminescent reporter of insulin secretion for 
            discovering regulators of pancreatic Beta-cell function.
  JOURNAL   Cell Metab. 2015 Jan 6;21(1):126-37. doi: 
            10.1016/j.cmet.2014.12.010.
  PUBMED    25565210
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2014.12"; journalName: "Cell Metab"; date: 
            "2015-01-6- 6"; volume: "21"; issue: "1"; pages: "126-37"
COMMENT     Sequence Label: Proinsulin-NanoLuc in pLX304
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             268..777
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
ORIGIN
        1 atggccctgt ggatgcgctt cctgcccctg ctggccctgc tcttcctctg ggagtcccac
       61 cccacccagg cttttgtcaa gcagcacctt tgtggttccc acctggtgga ggctctctac
      121 ctggtgtgtg gggagcgtgg cttcttctac acacccatgt cccgccgtga agtggaggac
      181 ccacaagtgg cacaactgga gctgggtgga ggcccgggag caggtgacct tcagaccttg
      241 gcactggagg tggcccagca gaagagggtc ttcacactcg aagatttcgt tggggactgg
      301 cgacagacag ccggctacaa cctggaccaa gtccttgaac agggaggtgt gtccagtttg
      361 tttcagaatc tcggggtgtc cgtaactccg atccaaagga ttgtcctgag cggtgaaaat
      421 gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg
      481 ggccagatcg aaaaaatttt taaggtggtg taccctgtgg atgatcatca ctttaaggtg
      541 atcctgcact atggcacact ggtaatcgac ggggttacgc cgaacatgat cgactatttc
      601 ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa agatcactgt aacagggacc
      661 ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca accccgacgg ctccctgctg
      721 ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat tctggcgaag
      781 cgtggcattg tagatcagtg ctgcaccagc atctgctccc tctaccagct ggagaactac
      841 tgcaactag
//
