LOCUS       sequence-101303-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRNA synthesis of bacterial nitroreductase NfsB codon modified for 
            zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Horstick EJ, Jordan DC, Bergeron SA, Tabor KM, Serpe M, Feldman B, 
            Burgess HA
  TITLE     Increased functional protein expression using nucleotide sequence 
            features enriched in highly expressed genes in zebrafish.
  JOURNAL   Nucleic Acids Res. 2015 Jan 27. pii: gkv035.
  PUBMED    25628360
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jan 27. pii: gkv035."
COMMENT     Sequence Label: pCS2-NfsB.zf1
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..81
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     63..80
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    802..936
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(826..845)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     880..899
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctatataagc aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct
       61 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat ccgccaccat
      121 ggatattatt agtgtggccc tgaagagaca tagcacaaag gcttttgatg ctagcaagaa
      181 gctgacacct gagcaggctg gacagatcaa gacactgctc cagtacagcc ctagcagcac
      241 aaacagccag ccttggcact tcattgtggc tagcacagag gagggaaagg ctagagtggc
      301 taagtctgct gctggaaact atgtgttcaa tgagagaaag atgctggatg ctagccatgt
      361 ggtggtgttc tgtgctaaga cagctatgga tgatgtgtgg ctgaagctgg tggtggacca
      421 ggaggatgct gatggaagat ttgctacacc tgaggctaag gctgctaatg acaagggaag
      481 aaagttcttt gctgacatgc acagaaagga cctgcatgat gatgctgagt ggatggctaa
      541 gcaggtgtac ctgaatgtgg gaaacttcct gctgggagtg gctgctctgg gactggatgc
      601 tgtgcctatt gagggatttg atgctgctat cctggatgct gagtttggac tgaaggagaa
      661 gggatacaca agcctggtgg tggtgcctgt gggacaccac tctgtggagg acttcaatgc
      721 tacactgcct aagagcagac tgcctcagaa catcacactg acagaggtgt gatctagaac
      781 tatagtgagt cgtattacgt agatccagac atgataagat acattgatga gtttggacaa
      841 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
      901 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
      961 atgtttcagg ttcaggggga ggtgtgggag gtttttt
//
