LOCUS       sequence-101310-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. Both 
            fragments are driven by PBAD. The C-terminal fragment has the point 
            mutation N748D. Contains a constitutive araC ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSara-N748D
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(724..741)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tctgatgttc actcagccga atcaggctgc tggatacatg gctaagctga tttgggaatc
       61 tgtgagcgtg acggtggtag ctgcggttga agcaatgaac tggcttaagt ctgctgctaa
      121 gctgctggct gctgaggtca aagataagaa gactggagag attcttcgca agcgttgcgc
      181 tgtgcattgg gtaactcctg atggtttccc tgtgtggcag gaatacaaga agcctattca
      241 gacgcgcttg gatctgatgt tcctcggtca gttccgctta cagcctacca ttaacaccaa
      301 caaagatagc gagattgatg cacacaaaca ggagtctggt atcgctccta actttgtaca
      361 cagccaagac ggtagccacc ttcgtaagac tgtagtgtgg gcacacgaga agtacggaat
      421 cgaatctttt gcactgattc acgactcctt cggtaccatt ccggctgacg ctgcgaacct
      481 gttcaaagca gtgcgcgaaa ctatggttga cacatatgag tcttgtgatg tactggctga
      541 tttctacgac cagttcgctg accagttgca cgagtctcaa ttggacaaaa tgccagcact
      601 tccggctaaa ggtaacttga acctccgtga catcttagag tcggacttcg cgttcgcgta
      661 atctagagtc gacctgcagg catgcaagct tggctgtttt ggcggatgag agaagatttt
      721 cagcctgata cagattaaat cagaacgcag aagc
//
