LOCUS       sequence-101312-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of flag-tagged PPAR gamma.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Hauser S, Adelmant G, Sarraf P, Wright HM, Mueller E, Spiegelman BM
  TITLE     Degradation of the peroxisome proliferator-activated receptor gamma 
            is linked to ligand-dependent activation.
  JOURNAL   J Biol Chem. 2000 Jun 16. 275(24):18527-33.
  PUBMED    10748014
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Jun 16. 275(24):18527-33."
COMMENT     Sequence Label: pcDNA flag PPAR gamma
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             184..207
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagtta agcttggtac cgagctcgga
      121 tccactagtc cagtgtggtg gaattctgca gatatccagc acagtggcgg ccgcaccacc
      181 atggactaca aagacgatga cgataaagca atgggtgaaa ctctgggaga ttctcctgtt
      241 gacccagagc atggtgcctt cgctgatgca ctgcctatga gcacttcaca agaaattacc
      301 atggttgaca cagagatgcc attctggccc accaacttcg gaatcagctc tgtggacctc
      361 tccgtgatgg aagaccactc gcattccttt gacatcaagc cctttaccac agttgatttc
      421 tccagcattt ctgctccaca ctatgaagac attccattca caagagctga cccaatggtt
      481 gctgattaca aatatgacct gaagctccaa gaataccaaa gtgcgatcaa agtagaacct
      541 gcatctccac cttattattc tgaaaagacc cagctctaca acaggcctca tgaagaacct
      601 tctaactccc tcatggccat tgagtgccga gtctgtgggg ataaagcatc aggcttccac
      661 tatggagttc atgcttgtga aggatgcaag ggttttttcc gaagaaccat ccgattgaag
      721 cttatttatg ataggtgtga tcttaactgc cggatccaca aaaaaagtag aaataaatgt
      781 cagtactgtc ggtttcagaa gtgccttgct gtggggatgt ctcacaatgc catcaggttt
      841 gggcggatgc cacaggccga gaaggagaag ctgttggcgg agatctccag tgatatcgac
      901 cagctgaacc cagagtctgc tgatctgcga gccctggcaa agcatttgta tgactcatac
      961 ataaagtcct tcccgctgac caaagccaag gcgagggcga tcttgacagg aaagacaacg
     1021 gacaaatcac catttgtcat ctacgacatg aattccttaa tgatgggaga agataaaatc
     1081 aagttcaaac atatcacccc
//
