LOCUS       sequence-101313-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of flag-tagged PPAR gamma.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Hauser S, Adelmant G, Sarraf P, Wright HM, Mueller E, Spiegelman BM
  TITLE     Degradation of the peroxisome proliferator-activated receptor gamma 
            is linked to ligand-dependent activation.
  JOURNAL   J Biol Chem. 2000 Jun 16. 275(24):18527-33.
  PUBMED    10748014
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Jun 16. 275(24):18527-33."
COMMENT     Sequence Label: pcDNA flag PPAR gamma
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(34..63)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atggtgatgg tgatgatgac cggtatgcat attcagatcc tcttctgaga tgagtttttg
       61 ttcgaagggc cctctagact aatacaagtc cttgtagatc tcctggagca gggggtgaag
      121 gctcatgtct gtctctgtct tcttgatcac atgcagtagc tgcacgtgct ctgtgacgat
      181 ctgcctgagg tctgtcatct tctggagcac cttggcgaac agctgagagg actctgggtg
      241 attcagcttg agctgcagtt ccagggcctg cagcaggttg tcttggatgt cctcgatggg
      301 cttcacgttc agcaagcctg ggcggtctcc actgagaata atgacagcta taaatatagc
      361 caagtcactg tcatctaatt ccagtgcatt gaacttcaca gcaaactcaa acttaggctc
      421 cataaagtca ccaaagggct tccgcaggct tttgaggaac tccctggtca tgaatccttg
      481 gccctctgag atgaggactc catctttatt catcagggag gccagcatcg tgtagatgat
      541 ctcatggaca ccatacttga gcagagtcac ttggtcattc aaatcaaggt taatgaaacc
      601 agggatattt ttggcatact ctgtgatctc ttgcacggct tctacggatc gaaactggca
      661 cccttgaaaa attcggatgg ccacctcttt gctctgctcc tgcagggggg tgatatgttt
      721 gaacttgatt ttatcttctc ccatcattaa ggaattcatg tcgtagatga caaatggtga
      781 tttgtccgtt gtctttcctg tcaagatcgc cctcgccttg gctttggtca gcgggaagga
      841 ctttatgtat gagtcataca aatgctttgc cagggctcgc agatcagcag actctgggtt
      901 cagctggtcg atatcactgg agatctccgc caacagcttc tccttctcgg cctgtggcat
      961 ccgcccaaac ctgatggcat tgtgagacat ccccacagca aggcacttct gaaaccgaca
     1021 gtactgacat ttatttctac tttttt
//
