LOCUS       sequence-101315-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. Both 
            fragments are driven by the arabinose inducible promoter PBAD. Also 
            contains a constitutive araC ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSara
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(718..735)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 gttcactcag ccgaatcagg ctgctggata catggctaag ctgatttggg aatctgtgag
       61 cgtgacggtg gtagctgcgg ttgaagcaat gaactggctt aagtctgctg ctaagctgct
      121 ggctgctgag gtcaaagata agaagactgg agagattctt cgcaagcgtt gcgctgtgca
      181 ttgggtaact cctgatggtt tccctgtgtg gcaggaatac aagaagccta ttcagacgcg
      241 cttgaacctg atgttcctcg gtcagttccg cttacagcct accattaaca ccaacaaaga
      301 tagcgagatt gatgcacaca aacaggagtc tggtatcgct cctaactttg tacacagcca
      361 agacggtagc caccttcgta agactgtagt gtgggcacac gagaagtacg gaatcgaatc
      421 ttttgcactg attcacgact ccttcggtac cattccggct gacgctgcga acctgttcaa
      481 agcagtgcgc gaaactatgg ttgacacata tgagtcttgt gatgtactgg ctgatttcta
      541 cgaccagttc gctgaccagt tgcacgagtc tcaattggac aaaatgccag cacttccggc
      601 taaaggtaac ttgaacctcc gtgacatctt agagtcggac ttcgcgttcg cgtaatctag
      661 agtcgacctg caggcatgca agcttggctg ttttggcgga tgagagaaga ttttcagcct
      721 gatacagatt aaatcagaac gcag
//
