LOCUS       sequence-101317-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome 
            editing in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Ryan OW, Skerker JM, Maurer MJ, Li X, Tsai JC, Poddar S, Lee ME, 
            DeLoache W, Dueber JE, Arkin AP, Cate JH
  TITLE     Selection of chromosomal DNA libraries using a multiplex CRISPR 
            system.
  JOURNAL   Elife. 2014 Aug 19;3. doi: 10.7554/eLife.03703.
  PUBMED    25139909
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-08-19"; volume: "3. doi"; pages: " 10.7554/eLife.03703"
COMMENT     Sequence Label: pCAS
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             407..427
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             443..466
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      470..659
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 atatttagct agtcattatg aaaagttgaa gggtagtcca gaagataacg aacaaaaaca
       61 attgtttgtg gagcagcata agcattattt agatgagatt attgagcaaa tcagtgaatt
      121 ttctaagcgt gttattttag cagatgccaa tttagataaa gttcttagtg catataacaa
      181 acatagagac aaaccaatac gtgaacaagc agaaaatatt attcatttat ttacgttgac
      241 gaatcttgga gctcccgctg cttttaaata ttttgataca acaattgatc gtaaacgata
      301 tacgtctaca aaagaagttt tagatgccac tcttatccat caatccatca ctggtcttta
      361 tgaaacacgc attgatttga gtcagctagg aggtgacggt ggagggccaa aaaagaaaag
      421 aaaagttgaa gatgcttctg ctcatcatca ccatcaccat caccattaaa tccgctctaa
      481 ccgaaaagga aggagttaga caacctgaag tctaggtccc tatttatttt tttatagtta
      541 tgttagtatt aagaacgtta tttatatttc aaatttttct tttttttctg tacagacgcg
      601 tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaagt
      661 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      721 ataggctccg cccccctgac gagcatcac
//
