LOCUS       sequence-101318-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome 
            editing in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Ryan OW, Skerker JM, Maurer MJ, Li X, Tsai JC, Poddar S, Lee ME, 
            DeLoache W, Dueber JE, Arkin AP, Cate JH
  TITLE     Selection of chromosomal DNA libraries using a multiplex CRISPR 
            system.
  JOURNAL   Elife. 2014 Aug 19;3. doi: 10.7554/eLife.03703.
  PUBMED    25139909
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-08-19"; volume: "3. doi"; pages: " 10.7554/eLife.03703"
COMMENT     Sequence Label: pCAS
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctcaaccaca caaacacata aacacataca aatacaaata caagcttatt tacttgacat
       61 cgcgcgatct tccactattc agcgccgtcc gccctctctc gtgttttttg tttacgcgac
      121 aactatgcga aatccggagc aacgggcaac cgtttgggga aagaccacac ccacgcgcga
      181 tcgccatggc aacgaggtcg cacacgcccc acacccagac ctccctgcga gcgggcatgg
      241 gtacaatgtc cccgttgcca cagacaccac ttcgtagcac agcgcagagc gtagcgtgtt
      301 gttgctgctg acaaaagaaa atttttctta gcaaagcaaa ggaggggaag cacgggcaga
      361 tagcaccgta ccataccctt ggaaactcga aatgaacgaa gcaggaaatg agagaatgag
      421 agttttgtag gtatatatag cggtagtgtt tgcgcgttac catcatcttc tggatctatc
      481 tattgttctt ttcctcatca ctttcccctt tttcgctctt cttcttgtct tttatttctt
      541 tctttttttt aattgttccc tcgattggct atctaccaaa gaatccaaac ttaatacacg
      601 tatttatttg tccaattacc atggataaga aatactcaat aggcttagat atcggcacaa
      661 atagcgtcgg atgggcggtg atcactgatg aatataaggt tccgtctaaa aagttcaagg
      721 ttctgggaaa tacagaccgc cacagtatca aaaaaaatct tatag
//
