LOCUS       sequence-101319-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome 
            editing in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Ryan OW, Skerker JM, Maurer MJ, Li X, Tsai JC, Poddar S, Lee ME, 
            DeLoache W, Dueber JE, Arkin AP, Cate JH
  TITLE     Selection of chromosomal DNA libraries using a multiplex CRISPR 
            system.
  JOURNAL   Elife. 2014 Aug 19;3. doi: 10.7554/eLife.03703.
  PUBMED    25139909
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-08-19"; volume: "3. doi"; pages: " 10.7554/eLife.03703"
COMMENT     Sequence Label: pCAS
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      70..267
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
       61 ctaatcagta ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt
      121 ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc
      181 ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat
      241 cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtg
      301 tcgaaaacga gctctcgaga acccttaata gtcgaacaag aagcaggcaa agtttagagc
      361 actgcccctc cgcactcaaa aaagaaaaaa ctaggaggaa aataaaattc tcaaccacac
      421 aaacacataa acacatacaa atacaaatac aagcttattt acttgacatc gcgcgatctt
      481 ccactattca gcgccgtccg ccctctctcg tgttttttgt ttacgcgaca actatgcgaa
      541 atccggagca acgggcaacc gtttggggaa agaccacacc cacgcgcgat cgccatggca
      601 acgaggtcgc acacgcccca cacccagacc tccctgcgag cgggcatggg tacaatgtcc
      661 ccgttgccac agacaccact tcgtagcaca gcgcagagcg tagcgtgttg ttgctgctga
      721 caaaag
//
