LOCUS       sequence-101325-         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-16a
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(418..474,475..672,673..684)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAMA"
ORIGIN
        1 gtcggcatac cgtttgtctt gtttggtatt gattgacgaa tgctcaaaaa taatctcatt
       61 aatgcttagc gcagtctctc tatcgcttct gaaccccggt gcacctgtgc cgaaacgcaa
      121 atggggaaac acccgctttt tggatgatta tgcattgtct ccacattgta tgcttccaag
      181 attctggtgg gaatactgct gatagcctaa cgttcatgat caaaatttaa ctgttctaac
      241 ccctacttga cagcaatata taaacagaag gaagctgccc tgtcttaaac cttttttttt
      301 atcatcatta ttagcttact ttcataattg cgactggttc caattgacaa gcttttgatt
      361 ttaacgactt ttaacgacaa cttgagaaga tcaaaaaaca actaattatt cgaaacgatg
      421 agatttcctt caatttttac tgctgtttta ttcgcagcat cctccgcatt agctgctcca
      481 gtcaacacta caacagaaga tgaaacggca caaattccgg ctgaagctgt catcggttac
      541 tcagatttag aaggggattt cgatgttgct gttttgccat tttccaacag cacaaataac
      601 gggttattgt ttataaatac tactattgcc agcattgctg ctaaagaaga aggggtatct
      661 ctagagaaaa gagaggccat ggccaaagtg a
//
